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Bilayer polymeric nanocapsules: A formulation approach for a thermostable and adjuvanted E. coli antigen vaccine

Authors :
José Crecente-Campo
Silvia Lorenzo-Abalde
María J. Alonso
Noemi Csaba
África González-Fernández
Jorge Blanco
Juan Marzoa
Azucena Mora
Source :
Journal of Controlled Release. 286:20-32
Publication Year :
2018
Publisher :
Elsevier BV, 2018.

Abstract

One of the strategies used to improve the immunogenicity of purified protein antigens has relied on their association with synthetic nanocarriers, which, in general, have functioned as simple antigen containers. Here, we present a more advanced strategy based on the design of an antigen nanocarrier at the molecular level. The nanocarrier is composed of a vitamin E oily core, surrounded by two layers: a first layer of chitosan and a second of dextran sulphate. The selected antigen, IutA protein from Escherichia coli, was harboured between the two polymeric layers. The final bilayer nanocapsules had a nanometric size (≈ 200 nm), a negative zeta potential (< -40 mV) and a good antigen association efficiency (≈ 70%). The bilayer architecture led to an improvement on the formulation stability and the controlled release of the associated antigen. Remarkably, after being administered to mice, bilayer nanocapsules elicited higher IgG levels than those obtained with antigen precipitated with Alum. Moreover, freeze-dried nanocapsules were stable at room temperature for, at least, 3 months. These promising data, in addition to their contribution to the development of an uropathogenic E. coli vaccine, has allowed us to validate these novel bilayer nanocapsules as adequate platforms for the delivery of protein antigens.

Details

ISSN :
01683659
Volume :
286
Database :
OpenAIRE
Journal :
Journal of Controlled Release
Accession number :
edsair.doi.dedup.....40997382a231594622b31a28a540e79d
Full Text :
https://doi.org/10.1016/j.jconrel.2018.07.018