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Bilayer polymeric nanocapsules: A formulation approach for a thermostable and adjuvanted E. coli antigen vaccine
- Source :
- Journal of Controlled Release. 286:20-32
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- One of the strategies used to improve the immunogenicity of purified protein antigens has relied on their association with synthetic nanocarriers, which, in general, have functioned as simple antigen containers. Here, we present a more advanced strategy based on the design of an antigen nanocarrier at the molecular level. The nanocarrier is composed of a vitamin E oily core, surrounded by two layers: a first layer of chitosan and a second of dextran sulphate. The selected antigen, IutA protein from Escherichia coli, was harboured between the two polymeric layers. The final bilayer nanocapsules had a nanometric size (≈ 200 nm), a negative zeta potential (< -40 mV) and a good antigen association efficiency (≈ 70%). The bilayer architecture led to an improvement on the formulation stability and the controlled release of the associated antigen. Remarkably, after being administered to mice, bilayer nanocapsules elicited higher IgG levels than those obtained with antigen precipitated with Alum. Moreover, freeze-dried nanocapsules were stable at room temperature for, at least, 3 months. These promising data, in addition to their contribution to the development of an uropathogenic E. coli vaccine, has allowed us to validate these novel bilayer nanocapsules as adequate platforms for the delivery of protein antigens.
- Subjects :
- 0301 basic medicine
Pharmaceutical Science
02 engineering and technology
Nanocapsules
Chitosan
Mice
03 medical and health sciences
chemistry.chemical_compound
Adjuvants, Immunologic
Antigen
Escherichia coli
Zeta potential
Animals
Vitamin E
Escherichia coli Infections
Escherichia coli Vaccines
Bilayer
Immunogenicity
Dextrans
021001 nanoscience & nanotechnology
Controlled release
Freeze Drying
RAW 264.7 Cells
030104 developmental biology
chemistry
Delayed-Action Preparations
Immunoglobulin G
Biophysics
Female
Nanocarriers
0210 nano-technology
Subjects
Details
- ISSN :
- 01683659
- Volume :
- 286
- Database :
- OpenAIRE
- Journal :
- Journal of Controlled Release
- Accession number :
- edsair.doi.dedup.....40997382a231594622b31a28a540e79d
- Full Text :
- https://doi.org/10.1016/j.jconrel.2018.07.018