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Polyphenolic fraction of Pilea microphylla (L.) protects Chinese hamster lung fibroblasts against γ-radiation-induced cytotoxicity and genotoxicity
- Source :
- Environmental toxicology and pharmacology. 33(1)
- Publication Year :
- 2011
-
Abstract
- Present study was designed to compare cytoprotective and antigenotoxic activity of the polyphenolic fraction of Pilea microphylla (PM1) with that of its active polyphenolic constituents against γ-radiation in V79 cells. PM1 was standardized with respect to the polyphenols present by RP-HPLC. It was evaluated for its free radical scavenging potential using Fenton reaction-induced DNA damage and lipid peroxidation. Further, PM1 was subjected against γ-radiation-induced cytotoxicity and genotoxicity in V79 cells. PM1 significantly reduced free radical-mediated calf thymus DNA damage and lipid peroxidation. Among the concentrations tested (12.5, 25 and 50 μg/ml) for radioprotection, PM1 at 25 μg/ml exhibited maximum protection. Further, when compared with constituent polyphenols viz., rutin, quercetin and chlorogenic acid (concentrations equivalent to that present in PM1-25 μg/ml), a combination of polyphenols was found most effective in preventing γ-radiation-induced cytotoxicity and genotoxicity. To conclude, radioprotection is possibly a synergistic effect of the phytochemicals present in the herbal extract, rather than any single component.
- Subjects :
- DNA damage
Health, Toxicology and Mutagenesis
Radiation-Protective Agents
Pharmacology
Toxicology
medicine.disease_cause
Cell Line
Lipid peroxidation
Rutin
chemistry.chemical_compound
Cricetulus
Cricetinae
medicine
TBARS
Organic chemistry
Animals
Humans
Cytotoxicity
Urticaceae
Lung
Chromatography, High Pressure Liquid
Micronucleus Tests
Plant Extracts
food and beverages
Polyphenols
General Medicine
Free Radical Scavengers
Fibroblasts
chemistry
Polyphenol
Gamma Rays
Comet Assay
Lipid Peroxidation
Quercetin
Genotoxicity
DNA Damage
Subjects
Details
- ISSN :
- 18727077
- Volume :
- 33
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Environmental toxicology and pharmacology
- Accession number :
- edsair.doi.dedup.....40910044472d1ff02fbb1527e6b63d01