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A Role for Circular Non-Coding RNAs in the Pathogenesis of Sporadic Parathyroid Adenomas and the Impact of Gender-Specific Epigenetic Regulation
- Source :
- Cells, Vol 8, Iss 1, p 15 (2018), Cells, Volume 8, Issue 1
- Publication Year :
- 2018
- Publisher :
- MDPI AG, 2018.
-
Abstract
- Epigenetic changes, including altered small non-coding RNAs, appear to be implicated in the pathogenesis of sporadic parathyroid adenomas (PAs). In this study, we investigated the circular RNAs (circRNAs) expression profile in sporadic PAs. Sixteen tissue samples of sporadic PAs, and four samples of normal parathyroid tissue (NPT) were investigated. Sample preparation and microarray hybridization were performed based on the Arraystar&rsquo<br />s standard protocols, and circRNAs sequences were predicted by bioinformatics tools. We identified 35 circRNAs that were differentially expressed in sporadic PAs compared to NPT<br />22 were upregulated, and 13 were downregulated, according to the pre-defined thresholds of fold-change &gt<br />2.0 and p &lt<br />0.05. In the subgroup analysis of PAs from male patients (n = 7) compared to PAs from female patients (n = 9), we also find a different expression profile. In particular, 19 circRNAs were significantly upregulated, and four circRNAs were significantly downregulated in male patients, compared to female counterparts. We show here for the first time a differential circRNA expression pattern in sporadic PAs compared to NPT, and a different expression profile in PA samples from male compared to female patients, suggesting an epigenetic role in the PA pathogenesis, and also an effect of gender in the epigenetic regulation of PAs.
- Subjects :
- Adenoma
Male
0301 basic medicine
RNA, Untranslated
Biology
parathyroid adenomas
Article
Epigenesis, Genetic
Pathogenesis
03 medical and health sciences
Sex Factors
Expression pattern
Downregulation and upregulation
Female patient
Humans
RNA, Neoplasm
Epigenetics
lcsh:QH301-705.5
Aged
epigenetics
Gene Expression Profiling
pathogenesis
RNA, Circular
General Medicine
Middle Aged
Molecular biology
Gene Expression Regulation, Neoplastic
Parathyroid Neoplasms
030104 developmental biology
lcsh:Biology (General)
Male patient
RNA
non-coding RNAs
Microarray hybridization
Female
circular RNAs
Subjects
Details
- ISSN :
- 20734409
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Cells
- Accession number :
- edsair.doi.dedup.....407f6ffce244e703070057f770805ccf