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A role for specific collagen motifs during wound healing and inflammatory response of fibroblasts in the teleost fish gilthead seabream
- Source :
- Molecular Immunology, Molecular Immunology, 2011, 48, pp.826-34. ⟨10.1016/j.molimm.2010.12.004⟩, Molecular Immunology, Elsevier, 2011, 48, pp.826-34. ⟨10.1016/j.molimm.2010.12.004⟩
- Publication Year :
- 2011
- Publisher :
- Elsevier BV, 2011.
-
Abstract
- International audience; Specific sites and sequences in collagen to which cells can attach, either directly or through protein intermediaries, were identified using Toolkits of 63-amino acid triple-helical peptides and specific shorter GXX'GEX″ motifs, which have different intrinsic affinity for integrins that mediate cell adhesion and migration. We have previously reported that collagen type I (COL-I) was able to prime in vitro the respiratory burst and induce a specific set of immune- and extracellular matrix-related molecules in phagocytes of the teleost fish gilthead seabream (Sparus aurata L.). It was also suggested that COL-I would provide an intermediate signal during the early inflammatory response in gilthead seabream. Since fibroblasts are highly involved in the initiation of wound repair and regeneration processes, in the present study SAF-1 cells (gilthead seabream fibroblasts) were used to identify the binding motifs in collagen by end-point and real-time cell adhesion assays using the collagen peptides and Toolkits. We identified the collagen motifs involved in the early magnesium-dependent adhesion of these cells. Furthermore, we found that peptides containing the GFOGER and GLOGEN motifs (where O is hydroxyproline) present high affinity for SAF-1 adhesion, expressed as both cell number and surface covering, while in cell suspensions, these motifs were also able to induce the expression of the genes encoding the proinflammatory molecules interleukin-1β and cyclooxygenase-2. These data suggest that specific collagen motifs are involved in the regulation of the inflammatory and healing responses of teleost fish.
- Subjects :
- MESH: Antigens, CD29
MESH: Inflammation
Amino Acid Motifs
Interleukin-1beta
MESH: Sea Bream
Integrin
MESH: Amino Acid Sequence
MESH: Receptors, Pattern Recognition
Extracellular matrix
MESH: Amino Acid Motifs
MESH: Structure-Activity Relationship
0302 clinical medicine
MESH: Interleukin-1beta
Sparus aurata
MESH: Collagen
MESH: Magnesium
MESH: Animals
Magnesium
Cyclooxygenase-2
MESH: Static Electricity
Regulation of gene expression
0303 health sciences
biology
MESH: Peptides
Protein Stability
Integrin beta1
MESH: Gene Expression Regulation
[SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM]
Interleukin-1β
Collagen motifs
Respiratory burst
Biochemistry
Receptors, Pattern Recognition
030220 oncology & carcinogenesis
Adhesion
Collagen
MESH: Cyclooxygenase 2
Molecular Sequence Data
Static Electricity
Immunology
Wound healing
Article
MESH: Cell Adhesion
Proinflammatory cytokine
Structure-Activity Relationship
03 medical and health sciences
MESH: Protein Stability
Cell Adhesion
Animals
Humans
Amino Acid Sequence
RNA, Messenger
Cell adhesion
Molecular Biology
MESH: RNA, Messenger
030304 developmental biology
Inflammation
MESH: Humans
MESH: Molecular Sequence Data
Fibroblasts
Sea Bream
In vitro
MESH: Wound Healing
Gene Expression Regulation
MESH: Fibroblasts
Cyclooxygenase 2
biology.protein
Peptides
Teleost fish
Interleukin-1
Subjects
Details
- ISSN :
- 01615890
- Volume :
- 48
- Database :
- OpenAIRE
- Journal :
- Molecular Immunology
- Accession number :
- edsair.doi.dedup.....4076f14b66af88603c935a5e1d349f32