Back to Search
Start Over
miRNA expression in anaplastic thyroid carcinomas
- Source :
- PLoS ONE, Vol 9, Iss 8, p e103871 (2014), PloS one, 9 (8, PLoS ONE
- Publication Year :
- 2014
- Publisher :
- Public Library of Science (PLoS), 2014.
-
Abstract
- Anaplastic thyroid carcinoma (ATC) is the most lethal form of thyroid neoplasia and represents an end stage of thyroid tumor progression. No effective treatment exists so far. In this study, we analyzed the miRNA expression profiles of 11 ATC by microarrays and their relationship with the mRNA expression profiles of the same 11 ATC samples. ATC show distinct miRNA expression profiles compared to other less aggressive thyroid tumor types. ATC show 18 commonly deregulated miRNA compared to normal thyroid tissue (17 downregulated and 1 upregulated miRNA). First, the analysis of a combined approach of the mRNA gene expression and of the bioinformatically predicted mRNA targets of the deregulated miRNA suggested a role for these regulations in the epithelial to mesenchymal transition (EMT) process in ATC. Second, the direct interaction between one of the upregulated mRNA target, the LOX gene which is an EMT key player, and a downregulated miRNA, the miR-29a, was experimentally validated by a luciferase assay in HEK cell. Third, we confirmed that the ATC tissue is composed of about 50% of tumor associated macrophages (TAM) and suggested, by taking into account our data and published data, their most likely direct or paracrine intercommunication between them and the thyroid tumor cells, amplifying the tumor aggressiveness. Finally, we demonstrated by in situ hybridization a specific thyrocyte localization of 3 of the deregulated miRNA: let-7g, miR-29a and miR-30e and we pointed out the importance of identifying the cell type localization before drawing any conclusion on the physiopathological role of a given gene.<br />SCOPUS: ar.j<br />info:eu-repo/semantics/published
- Subjects :
- Pathology
DNA Mutational Analysis
Thyroid Gland
lcsh:Medicine
Thyroid Carcinoma, Anaplastic
Endocrinology
Cell Signaling
Gene expression
Medicine and Health Sciences
Endocrine Tumors
lcsh:Science
Anaplastic Thyroid Carcinoma
In Situ Hybridization
Regulation of gene expression
Multidisciplinary
Thyroid
Gene Therapy
Sciences bio-médicales et agricoles
Up-Regulation
Gene Expression Regulation, Neoplastic
medicine.anatomical_structure
Oncology
Transcriptome Analysis
Research Article
Signal Transduction
medicine.medical_specialty
Epithelial-Mesenchymal Transition
Down-Regulation
Biology
Carcinomas
Thyroid carcinoma
Diagnostic Medicine
microRNA
Biomarkers, Tumor
medicine
Humans
RNA, Messenger
Thyroid Neoplasms
Epithelial–mesenchymal transition
Molecular Biology Techniques
Molecular Biology
HEK 293 cells
lcsh:R
Computational Biology
Biology and Life Sciences
Cancers and Neoplasms
Cell Biology
Genome Analysis
MicroRNAs
Tumor progression
Cancer research
lcsh:Q
Thyroid Carcinomas
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 9
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....4074ad93047467f509eb77dab86ab86c