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Characterization of hemicelluloses from Neolamarckia cadamba (Rubiaceae) during xylogenesis
- Source :
- Carbohydrate polymers. 156
- Publication Year :
- 2016
-
Abstract
- In this study, hemicelluloses were isolated from the apical, middle and basal segments of N. cadamba using a 2N KOH extraction procedure. Chemical composition and structural characterization of the three hemicellulosic fractions obtained were comparatively investigated by a combination of HPLC, GPC, FTIR, 1H,13C, HSQC NMR and TGA techniques. According to the sugar analysis and spectral results, (4-O-methyl) glucuronoxylan was the primary hemicellulose identified in the samples with trace levels of mannan also present. All of the three samples showed spherical polymers in 0.005M sodium phosphate solution (containing 0.02N NaCl). Xylan in the middle and basal segment stems had higher molecular weights. Our findings show that during xylogenesis in N. cadamba, changes are observed in xylan content and molecular weight.
- Subjects :
- 0106 biological sciences
food.ingredient
Magnetic Resonance Spectroscopy
Polymers and Plastics
Neolamarckia
Rubiaceae
02 engineering and technology
01 natural sciences
chemistry.chemical_compound
food
Polysaccharides
010608 biotechnology
Glucuronoxylan
Materials Chemistry
Organic chemistry
Hemicellulose
Sugar
Mannan
chemistry.chemical_classification
Chromatography
Molecular mass
Chemistry
Organic Chemistry
Extraction (chemistry)
021001 nanoscience & nanotechnology
Xylan
Molecular Weight
Xylans
0210 nano-technology
Subjects
Details
- ISSN :
- 18791344
- Volume :
- 156
- Database :
- OpenAIRE
- Journal :
- Carbohydrate polymers
- Accession number :
- edsair.doi.dedup.....4061641e588619e993e7eb1c376fc4c4