Supplementary Data from Organelle-Derived Acetyl-CoA Promotes Prostate Cancer Cell Survival, Migration, and Metastasis via Activation of Calmodulin Kinase II

This file contains immunofluorescence of LNCaP, C4-2B4, PC3, and PC3-mm2 PCa cell lines (Supplementary Fig.S1); Immunohistochemistry for the expression of pCaMKII (T286) in specimens from human primary prostate tumor, lymph node metastasis, and bone metastasis (Supplementary Fig. S2); Re-expression of constitutively active CaMKII in PC3-mm2 cells with knockout of CaMKII (Supplementary Fig. S3); Effect of re-expression of constitutively active CaMKII in PC3-mm2 cells with knockout of CaMKII on prostate cancer metastasis (Supplementary Fig. S4); Knockdown of AcoxI in PC3-mm2 cells (Supplementary Fig. S5); Effect of AcoxI-III knockout on the levels of individuallipid components (Supplementary Fig. S6) and Sequence of primers and gDNA of CaMKII and ACOX (Table S1).