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Testican 1 (SPOCK1) and protein tyrosine phosphatase, receptor type S (PTPRS) show significant increase in saliva of tobacco users with oral cancer
- Source :
- Translational Research in Oral Oncology, Vol 3 (2018)
- Publication Year :
- 2018
- Publisher :
- SAGE Publications, 2018.
-
Abstract
- Objectives: To identify potential candidate proteins which are secretory in nature and present at a higher abundance in oral cancer patients with tobacco habits. Methods: Conditioned media of tobacco-treated and -untreated non-neoplastic oral keratinocytes were analyzed using iTRAQ-based mass spectrometry. Hypersecreted proteins; SPARC (osteonectin), cwcv and kazal like domains proteoglycan 1 (SPOCK1); prosaposin (PSAP); and protein tyrosine phosphatase, receptor type S (PTPRS) were validated by enzyme-linked immunosorbent assay (ELISA) using saliva samples from oral cancer patients who are tobacco users. Results: Proteomic analysis of tobacco-treated and -untreated cells led to the identification of 2873 proteins. Among these, 378 proteins showed high abundance and 253 proteins showed low abundance (2-fold cutoff) in conditioned-media of tobacco-treated cells. ELISA-based validation showed significantly higher levels of SPOCK1, PSAP, and PTPRS in oral cancer patients with tobacco chewing habits compared to healthy controls. However, PSAP showed low specificity compared to SPOCK1 and PTPRS. Conclusions: This study indicates significantly increased levels of SPOCK1, PSAP, and PTPRS in saliva of oral cancer patients with tobacco habits. These protein biomarkers might be useful to identify tobacco users with high risk of developing oral cancers.
- Subjects :
- 0301 basic medicine
Saliva
business.industry
Head and neck cancer
lcsh:Surgery
Cancer
lcsh:RD1-811
Protein tyrosine phosphatase
Receptor type
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
medicine.disease
lcsh:RC254-282
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
Smokeless tobacco
Tobacco users
030220 oncology & carcinogenesis
Cancer research
medicine
PTPRS
business
Subjects
Details
- ISSN :
- 2057178X
- Volume :
- 3
- Database :
- OpenAIRE
- Journal :
- Translational Research in Oral Oncology
- Accession number :
- edsair.doi.dedup.....4034255b286081e764fd4b7696cf51e9