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Simple and Rapid Method for Wogonin Preparation and Its Biotransformation
- Source :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences, Vol 22, Iss 8973, p 8973 (2021), Volume 22, Issue 16
- Publication Year :
- 2021
- Publisher :
- MDPI, 2021.
-
Abstract
- Wogonin is one of the most active flavonoids from Scutellaria baicalensis Georgi (baikal skullcap), widely used in traditional Chinese medicine. It exhibits a broad spectrum of health-promoting and therapeutic activities. Together with baicalein, it is considered to be the one of main active ingredients of Chinese medicines for the management of COVID-19. However, therapeutic use of wogonin may be limited due to low market availability connected with its low content in baikal skullcap and lack of efficient preparative methods for obtaining this compound. Although the amount of wogonin in skullcap root often does not exceed 0.5%, this material is rich in wogonin glucuronide, which may be used as a substrate for wogonin production. In the present study, a rapid, simple, cheap and effective method of wogonin and baicalein preparation, which provides gram quantities of both flavonoids, is proposed. The obtained wogonin was used as a substrate for biotransformation. Thirty-six microorganisms were tested in screening studies. The most efficient were used in enlarged scale transformations to determine metabolism of this xenobiotic. The major phase I metabolism product was 4′-hydroxywogonin—a rare flavonoid which exhibits anticancer activity—whereas phase II metabolism products were glucosides of wogonin. The present studies complement and extend the knowledge on the effect of substitution of A- and B-ring on the regioselective glycosylation of flavonoids catalyzed by microorganisms.
- Subjects :
- baicalein
QH301-705.5
Radix Scutellariae
Flavonoid
Microbial Sensitivity Tests
wogonin
Plant Roots
Catalysis
Article
Inorganic Chemistry
chemistry.chemical_compound
Wogonin
Biotransformation
baikal skullcap
Animals
Humans
Physical and Theoretical Chemistry
Biology (General)
Molecular Biology
QD1-999
Spectroscopy
chemistry.chemical_classification
Active ingredient
biology
Plant Extracts
SARS-CoV-2
Organic Chemistry
Fungi
Scutellaria baicalensis
General Medicine
biology.organism_classification
Computer Science Applications
Baicalein
COVID-19 Drug Treatment
Chemistry
chemistry
Biochemistry
Flavanones
biotransformation
Glucuronide
isolation
Drug metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Volume :
- 22
- Issue :
- 16
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....401bd6fccc07cca2e4d004a7ac03dbd5