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Molecular Threading: Mechanical Extraction, Stretching and Placement of DNA Molecules from a Liquid-Air Interface
- Source :
- PLoS ONE, Vol 8, Iss 7, p e69058 (2013), PLoS ONE
- Publication Year :
- 2013
- Publisher :
- Public Library of Science (PLoS), 2013.
-
Abstract
- We present "molecular threading", a surface independent tip-based method for stretching and depositing single and double-stranded DNA molecules. DNA is stretched into air at a liquid-air interface, and can be subsequently deposited onto a dry substrate isolated from solution. The design of an apparatus used for molecular threading is presented, and fluorescence and electron microscopies are used to characterize the angular distribution, straightness, and reproducibility of stretched DNA deposited in arrays onto elastomeric surfaces and thin membranes. Molecular threading demonstrates high straightness and uniformity over length scales from nanometers to micrometers, and represents an alternative to existing DNA deposition and linearization methods. These results point towards scalable and high-throughput precision manipulation of single-molecule polymers.
- Subjects :
- Technology
lcsh:Medicine
Condensed Matter
02 engineering and technology
Engineering
Nanotechnology
Nanobiotechnology
lcsh:Science
Condensed-Matter Physics
chemistry.chemical_classification
0303 health sciences
Multidisciplinary
Physics
Air
Robotics
Polymer
021001 nanoscience & nanotechnology
Condensed Matter Fluids
Nanorobotics
Solutions
Membrane
Transmission electron microscopy
Microtechnology
Optoelectronics
0210 nano-technology
Research Article
Materials science
Surface Properties
Materials Science
DNA, Single-Stranded
Bioengineering
Biomaterials
03 medical and health sciences
Molecule
Thin film
030304 developmental biology
business.industry
Mechanical Engineering
lcsh:R
Reproducibility of Results
DNA
Microscopy, Electron
Microscopy, Fluorescence
chemistry
Nanoengineering
Bionanotechnology
Biophysics
Nucleic Acid Conformation
lcsh:Q
Nanometre
Threading (protein sequence)
business
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....4017f6d01e790a8a1c7b6af3822e49ed