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Packaging of Tobacco Mosaic Virus Subgenomic RNAs by Brome Mosaic Virus Coat Protein Exhibits RNA Controlled Polymorphism
- Source :
- Virology. 275(2):249-257
- Publication Year :
- 2000
- Publisher :
- Elsevier BV, 2000.
-
Abstract
- The coat protein (CP) of icosahedral Brome mosaic virus (BMV) was expressed from a genetically engineered rod-shape Tobacco mosaic virus. Molecular characterization of the progeny recovered from symptomatic plants revealed that BMV CP selectively packaged the three subgenomic RNAs of the hybrid virus into two differently sized icosahedral virus-like particles (VLPs). The smaller VLPs packaged only the two smaller subgenomic RNAs. Additional in vitro reassembly assays with BMV CP subunits and transcripts of hybrid subgenomic RNAs further demonstrated that the ability of BMV capsids to display polymorphism is not dependent on the RNA size alone and appears to be controlled by some other feature(s) of the genetically engineered RNA.
- Subjects :
- Xenopus
viruses
Chenopodiaceae
Biology
Virus
Capsid
Brome mosaic virus
Plant virus
Virology
Tobacco mosaic virus
Animals
Subgenomic mRNA
Polymorphism, Genetic
Virus Assembly
Virion
RNA
food and beverages
Blotting, Northern
biology.organism_classification
Bromovirus
Molecular biology
In vitro
Tobacco Mosaic Virus
RNA, Viral
Protein Binding
Subjects
Details
- ISSN :
- 00426822
- Volume :
- 275
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Virology
- Accession number :
- edsair.doi.dedup.....400f5851291d75e46b15649f58b2e73a
- Full Text :
- https://doi.org/10.1006/viro.2000.0532