Back to Search
Start Over
Chromosome-biased binding and gene regulation by the Caenorhabditis elegans DRM complex
- Source :
- PLoS Genetics, PLoS, PLoS Genetics, Vol 7, Iss 5, p e1002074 (2011)
- Publication Year :
- 2010
-
Abstract
- DRM is a conserved transcription factor complex that includes E2F/DP and pRB family proteins and plays important roles in development and cancer. Here we describe new aspects of DRM binding and function revealed through genome-wide analyses of the Caenorhabditis elegans DRM subunit LIN-54. We show that LIN-54 DNA-binding activity recruits DRM to promoters enriched for adjacent putative E2F/DP and LIN-54 binding sites, suggesting that these two DNA–binding moieties together direct DRM to its target genes. Chromatin immunoprecipitation and gene expression profiling reveals conserved roles for DRM in regulating genes involved in cell division, development, and reproduction. We find that LIN-54 promotes expression of reproduction genes in the germline, but prevents ectopic activation of germline-specific genes in embryonic soma. Strikingly, C. elegans DRM does not act uniformly throughout the genome: the DRM recruitment motif, DRM binding, and DRM-regulated embryonic genes are all under-represented on the X chromosome. However, germline genes down-regulated in lin-54 mutants are over-represented on the X chromosome. We discuss models for how loss of autosome-bound DRM may enhance germline X chromosome silencing. We propose that autosome-enriched binding of DRM arose in C. elegans as a consequence of germline X chromosome silencing and the evolutionary redistribution of germline-expressed and essential target genes to autosomes. Sex chromosome gene regulation may thus have profound evolutionary effects on genome organization and transcriptional regulatory networks.<br />National Institutes of Health (U.S.) (grant GM24663)<br />National Institutes of Health (U.S.) (grant DK068429)<br />National Institutes of Health (U.S.) (grant GM082971)<br />National Institutes of Health (U.S.) (grant GM076378)
- Subjects :
- Cancer Research
lcsh:QH426-470
DNA transcription
Transcription factor complex
Gene Expression
Germline
Chromosomes
Molecular Genetics
03 medical and health sciences
Model Organisms
Molecular cell biology
X Chromosome Inactivation
Genetics
Animals
Gene Regulation
DRM complex
Caenorhabditis elegans Proteins
Molecular Biology
Gene
Biology
Genome Evolution
Genetics (clinical)
Ecology, Evolution, Behavior and Systematics
X chromosome
Caenorhabditis elegans
030304 developmental biology
Regulation of gene expression
0303 health sciences
biology
Chromosome Biology
030302 biochemistry & molecular biology
Genomics
Animal Models
biology.organism_classification
3. Good health
lcsh:Genetics
Gene Expression Regulation
Caenorhabditis Elegans
Gene Function
Genome Expression Analysis
Chromatin immunoprecipitation
Transcription Factors
Research Article
Subjects
Details
- ISSN :
- 15537404
- Volume :
- 7
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- PLoS genetics
- Accession number :
- edsair.doi.dedup.....40064fc8b9767e944ee7ffe90ad1d81c