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MicroRNA in the ovine mammary gland during early pregnancy: spatial and temporal expression of miR-21, miR-205, and miR-200
- Source :
- Physiological Genomics 4 (45), 151-161. (2013), Physiological Genomics, Physiological Genomics, American Physiological Society, 2013, 45 (4), pp.151-161. ⟨10.1152/physiolgenomics.00091.2012⟩
- Publication Year :
- 2013
-
Abstract
- Chantier qualité GA; The mammary gland undergoes extensive remodeling between the beginning of pregnancy and lactation; this involves cellular processes including cell proliferation, differentiation, and apoptosis, all of which are under the control of numerous regulators. To unravel the role played by miRNA, we describe here 47 new ovine miRNA cloned from mammary gland in early pregnancy displaying strong similarities with those already identified in the cow, human, or mouse. A microarray study of miRNA variations in the adult ovine mammary gland during pregnancy and lactation showed that 100 miRNA are regulated according to three principal patterns of expression: a decrease in early pregnancy, a peak at midpregnancy, or an increase throughout late pregnancy and lactation. One miRNA displaying each pattern (miR-21, miR-205, and miR-200b) was analyzed by qRT-PCR. Variations in expression were confirmed for all three miRNA. Using in situ hybridization, we detected both miR-21 and miR-200 in luminal mammary epithelial cells when expressed, whereas miR-205 was expressed in basal cells during the first half of pregnancy and then in luminal cells during the second half. We therefore conclude that miR-21 is strongly expressed in the luminal cells of the normal mammary gland during early pregnancy when extensive cell proliferation occurs. In addition, we show that miR-205 and miR-200 are coexpressed in luminal cells, but only during the second half of pregnancy. These two miRNA may cooperate to maintain epithelial status by repressing an EMT-like program, to achieve and preserve the secretory phenotype of mammary epithelial cells.
- Subjects :
- mammary gland
Time Factors
Microarray
Physiology
expression d'arn
Mammary gland
immunohistochimie
Mice
0302 clinical medicine
ovin
Lactation
In Situ Hybridization
Oligonucleotide Array Sequence Analysis
Animal biology
0303 health sciences
Reverse Transcriptase Polymerase Chain Reaction
[SDV.BA]Life Sciences [q-bio]/Animal biology
Gene Expression Regulation, Developmental
brebis
Immunohistochemistry
Phenotype
Cell biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
Female
pregnancy
mirna
medicine.medical_specialty
mouton
In situ hybridization
Biology
arn non codant
03 medical and health sciences
Mammary Glands, Animal
gestation
Internal medicine
development
ovine
noncoding rna
microRNA
Biologie animale
Genetics
medicine
Animals
030304 developmental biology
Pregnancy
Sheep
Cell growth
Gene Expression Profiling
Keratin-8
Keratin-14
Epithelial Cells
medicine.disease
MicroRNAs
Endocrinology
glande mammaire
Cattle
hybridation in situ
Subjects
Details
- Language :
- English
- ISSN :
- 10948341 and 15312267
- Database :
- OpenAIRE
- Journal :
- Physiological Genomics 4 (45), 151-161. (2013), Physiological Genomics, Physiological Genomics, American Physiological Society, 2013, 45 (4), pp.151-161. ⟨10.1152/physiolgenomics.00091.2012⟩
- Accession number :
- edsair.doi.dedup.....4001f9c2a2aafd8e6f5ec4b48116a183