Fast Screening of Single-Nucleotide Polymorphisms Using Chip-Based Temperature Gradient Capillary Electrophoresis

Recently, the analysis of single-nucleotide polymorphisms has attracted much attention. Although many techniques have been reported, new methods with high resolving power, low-cost and fast speed are still in demand. We present a fast SNP detection scheme using chip-based temperature gradient capillary electrophoresis to separate the homoduplex and heteroduplex PCR products which contain one or two SNP sites. The total time of a single run was only 8 minutes.