Back to Search
Start Over
Expression and Secretion of an Atrial Natriuretic Peptide in Beige-Like 3T3-L1 Adipocytes
- Source :
- International Journal of Molecular Sciences, Volume 20, Issue 24
- Publication Year :
- 2019
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2019.
-
Abstract
- The browning of white adipose tissue (beige adipocytes) stimulates energy expenditure. Omega-3 fatty acids have been shown to induce thermogenic action in adipocytes via G-protein coupled receptor 120 (GPR120). Atrial natriuretic peptide (ANP) is a peptide hormone that plays the role of maintaining normal blood pressure in kidneys to inhibit Na+ reuptake. Recently, ANP was found to induce adipocyte browning by binding to NPR1, an ANP receptor. However, the expression of ANP in adipocytes has not yet been studied. Therefore, in this study, we investigate the expression of ANP in beige-like adipocytes induced by docosahexaenoic acids (DHA), T3, or a PPAR agonist, rosiglitazone. First, we found that brown adipocyte-specific genes were upregulated in beige-like adipocytes. DHA promoted ANP expression in beige-like cells, whereas DHA-induced ANP expression was abolished by GPR120 knockout. ANP secretion of beige-like adipocytes was increased via PKC/ERK1/2 signaling in the GPR120 pathway. Furthermore, ANP secreted from beige-like adipocytes acted on HEK-293 cells, the recipient cells, leading to increased cGMP activity. After the NPR1 knockdown of HEK-293 cells, cGMP activity was not changed. Taken together, our findings indicate that beige-like adipocytes induce ANP secretion, which may contribute to improving obesity-associated metabolic disease.
- Subjects :
- medicine.medical_specialty
MAP Kinase Signaling System
Peroxisome Proliferator-Activated Receptors
White adipose tissue
Peptide hormone
Catalysis
Article
Inorganic Chemistry
chemistry.chemical_compound
Gene Knockout Techniques
Mice
Atrial natriuretic peptide
Internal medicine
Adipocyte
3T3-L1 Cells
atrial natriuretic peptide
medicine
Animals
Adipocytes, Beige
Physical and Theoretical Chemistry
Receptor
Molecular Biology
Cyclic GMP
Spectroscopy
Protein Kinase C
Mitogen-Activated Protein Kinase 6
docosahexaenoic acids
Mitogen-Activated Protein Kinase 3
Chemistry
Organic Chemistry
GPR120
3T3-L1
General Medicine
NPR1
Computer Science Applications
Endocrinology
Gene Expression Regulation
cardiovascular system
beige adipocytes
Receptors, Atrial Natriuretic Factor
Atrial Natriuretic Factor
hormones, hormone substitutes, and hormone antagonists
circulatory and respiratory physiology
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....3ff9bb363bd6dcb47f4df30a4dc31032
- Full Text :
- https://doi.org/10.3390/ijms20246128