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Gut bacteria associated with different diets in reared Nephrops norvegicus
- Source :
- Systematic and Applied Microbiology. 35:473-482
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- The impact of different diets on the gut microbiota of reared Nephrops norvegicus was investigated based on bacterial 16S rRNA gene diversity. Specimens were collected from Pagasitikos Gulf (Greece) and kept in experimental rearing tanks, under in situ conditions, for 6months. Treatments included three diets: frozen natural (mussel) food (M), dry formulated pellet (P) and starvation (S). Gut samples were collected at the initiation of the experiment, and after 3 and 6months. Tank water and diet samples were also analyzed for bacterial 16S rRNA gene diversity. Statistical analysis separated the two groups fed or starved (M and P vs. S samples). Most gut bacteria were not related to the water or diet bacteria, while bacterial diversity was higher in the starvation samples. M and P samples were dominated by Gammaproteobacteria, Epsilonproteobacteria and Tenericutes. Phylotypes clustering in Photobacterium leiognathi, Shewanella sp. and Entomoplasmatales had high frequencies in the M and P samples but low sequence frequencies in S samples. The study showed that feeding resulted in the selection of specific species, which also occurs in the natural population, and might be associated with the animal's nutrition.
- Subjects :
- DNA, Bacterial
Molecular Sequence Data
Gut flora
DNA, Ribosomal
Applied Microbiology and Biotechnology
Microbiology
Entomoplasmatales
Photobacterium leiognathi
Nephrops norvegicus
RNA, Ribosomal, 16S
Gammaproteobacteria
Animals
Cluster Analysis
Food science
Phylogeny
Ecology, Evolution, Behavior and Systematics
Tenericutes
Epsilonproteobacteria
Bacteria
Greece
biology
Biodiversity
Sequence Analysis, DNA
biology.organism_classification
Diet
Nephropidae
Gastrointestinal Tract
Subjects
Details
- ISSN :
- 07232020
- Volume :
- 35
- Database :
- OpenAIRE
- Journal :
- Systematic and Applied Microbiology
- Accession number :
- edsair.doi.dedup.....3ff192e71c884b33c4a0865807eeb09c