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Cardiac Malformations Are Associated with Altered Expression of Vascular Endothelial Growth Factor and Endothelial Nitric Oxide Synthase Genes in Embryos of Diabetic Mice
- Source :
- Experimental Biology and Medicine. 233:1421-1432
- Publication Year :
- 2008
- Publisher :
- SAGE Publications, 2008.
-
Abstract
- The aim of this study was to investigate the role of nitric oxide (NO), and the expression of endothelial nitric oxide synthase (eNOS) and vascular endothelial growth factor (VEGF) genes in developing hearts at embryonic day 13.5 of embryos from diabetic mice. The protein and mRNA expression levels of eNOS and VEGF were significantly altered in the developing hearts of embryos from diabetic mice. The NO level was significantly decreased, whereas the VEGF concentration was significantly increased in the developing hearts of the embryos from diabetic mice. In vitro study showed a significant reduction in eNOS expression and cell proliferation in cardiac myoblast cells exposed to high glucose concentrations. Further, high glucose induced apoptosis in myoblast cells. Ultrastructural changes characteristics of apoptosis, including cell blebbing, aggregation of ribosomes and vacuoles in the cytoplasm were also evident in myoblast cells exposed to high glucose. It is suggested that hyperglycemia alters the expression of eNOS and VEGF genes that are involved in the regulation of cell growth and vasculogenesis, thereby contributing to the cardiac malformations seen in embryos from diabetic mice.
- Subjects :
- Heart Defects, Congenital
Vascular Endothelial Growth Factor A
medicine.medical_specialty
Embryonic Development
Apoptosis
General Biochemistry, Genetics and Molecular Biology
Diabetes Mellitus, Experimental
Nitric oxide
Mice
chemistry.chemical_compound
Vasculogenesis
Enos
Internal medicine
medicine
Animals
RNA, Messenger
Cells, Cultured
Cell Proliferation
biology
Cell growth
Myocardium
Heart
biology.organism_classification
Rats
Vascular endothelial growth factor
Nitric oxide synthase
Vascular endothelial growth factor A
Glucose
Endocrinology
chemistry
biology.protein
Female
Nitric Oxide Synthase
Myoblasts, Cardiac
Subjects
Details
- ISSN :
- 15353699 and 15353702
- Volume :
- 233
- Database :
- OpenAIRE
- Journal :
- Experimental Biology and Medicine
- Accession number :
- edsair.doi.dedup.....3fe90dd5b63db4969c1b8e398c86d877
- Full Text :
- https://doi.org/10.3181/0806-rm-186