A Novel Protocol for the Preparation of Sodium Tanshinone Sulphonates by Direct Ultrasound-assisted Sulphonation of the Crude Extract of the Roots of Salvia miltiorrhiza Bunge and Following Counter-current Chromatography Purification

Introduction Sodium tanshinone sulphonates are water-soluble derivatives of tanshinones originated from Tanshen (or Danshen, Salvia miltiorrhiza Bunge), a famous Traditional Chinese Medicine, which have potent biological activities, especially in the treatment of cardiovascular disorders. However, the classical preparation processes of sodium tanshinone sulphonates often involve multiple time- and solvent-consuming steps after purification of tanshinones, resulting in relatively low yields. Objective To develop a simple protocol for direct preparation of sodium tanshinone sulphonates from the complex crude extract of the roots of S. miltiorrhiza without pre-purification of tanshinones. Methodology The 100 mg crude tanshinone extract of S. miltiorrhiza was first sulphonated in a ultrasound bath with glacial acetic acid, acetic anhydride and concentrated sulphuric acid for 20 min, and then subjected to counter-current chromatography (CCC) separation using a optimum two-phase solvent system composed of n-hexane:ethylacetate:ethanol:5% sodium chloride aqueous solution (1:8:4:10, v/v). Based on the UV detection and HPLC analyses, the sulphonated fractions were collected. Result Sodium tanshinone IIA sulphonate (7.1 mg) and sodium tanshinone I sulphonate (2.8 mg) with over 95% purity were obtained successfully for the first time by ultrasound-assisted sulphonation and following CCC purification. Conclusion The study has shown that the method combining ultrasound-assisted sulphonation and CCC purification is an efficient way to prepare tanshinone sulphonates without pre-purification of tanshinones from the complex extracts of Tanshen, and can be explored as a new protocol for wide natural product modification directly from a crude complex extracts without pre-purification. Copyright © 2011 John Wiley & Sons, Ltd.