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Establishment and characterization of pygmy killer whale (Feresa attenuata) dermal fibroblast cell line
- Source :
- PLoS ONE, PLoS ONE, Vol 13, Iss 3, p e0195128 (2018)
- Publication Year :
- 2017
-
Abstract
- The pygmy killer whale (Feresa attenuata) (PKW) is a tropical and subtropical marine mammal commonly found in the Atlantic, Indian and Pacific oceans. Since the PKWs live in offshore protected territories, they are rarely seen onshore. Hence, PKW are one of the most poorly understood oceanic species of odontocetes. The dermal tissue comes primarily from stranding events that occur along the coast of the Shantou, Guangdong, China. The sampled tissues were immediately processed and attached on collagen-coated 6-well tissue culture plate. The complete medium (DMEM and Ham’s F12, fetal bovine serum, antibiotic and essential amino acids) was added to the culture plates. The primary culture (PKW-LWH) cells were verified as fibroblast by vimentin and karyotype analyses, which revealed 42 autosomes and two sex chromosomes X and Y. Following transfection of PKW-LWH cells with a plasmid encoding, the SV40 large T-antigens and the transfected cells were isolated and expanded. Using RT-PCR, western blot, immunofluorescence analysis and SV40 large T-antigen stability was confirmed. The cell proliferation rate of the fibroblast cells, PKW-LWHT was faster than the primary cells PKW-LWH with the doubling time 68.9h and 14.4h, respectively. In this study, we established PKW dermal fibroblast cell line for the first time, providing a unique opportunity for in vitro studies on the effects of environmental pollutants and pathogens that could be determined in PKW and/or Cetaceans.
- Subjects :
- 0301 basic medicine
Male
Cell Lines
Cell Culture Techniques
lcsh:Medicine
Marine and Aquatic Sciences
Pathology and Laboratory Medicine
Biochemistry
Tissue culture
0302 clinical medicine
Animal Cells
Medicine and Health Sciences
Electrochemistry
lcsh:Science
Cells, Cultured
Connective Tissue Cells
Mammals
Multidisciplinary
C100
Eukaryota
Karyotype
Killer Whales
Dermis
C900
Sv40
Chemistry
medicine.anatomical_structure
Connective Tissue
Medical Microbiology
030220 oncology & carcinogenesis
Viral Pathogens
Viruses
Physical Sciences
Vertebrates
Biological Cultures
Cellular Types
Anatomy
Pathogens
Research Article
Dolphins
Marine Biology
Biology
Transfection
Research and Analysis Methods
Microbiology
Dermal fibroblast
03 medical and health sciences
biology.animal
Primary Cells
medicine
Animals
Vimentin
Fibroblast
Molecular Biology Techniques
Marine Mammals
Molecular Biology
Microbial Pathogens
Whale
lcsh:R
Organisms
Whales
Biology and Life Sciences
Proteins
Cell Biology
Fibroblasts
Cell Cultures
biology.organism_classification
Molecular biology
Feresa attenuata
Polyomaviruses
Cytoskeletal Proteins
030104 developmental biology
Biological Tissue
Electrochemical Cells
Cell culture
Amniotes
Earth Sciences
lcsh:Q
Cultured Fibroblasts
DNA viruses
Fetal bovine serum
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 13
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....3fd6c637eebc86e0eba2876d433142d5