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Tissue memory B cell repertoire analysis after ALVAC/AIDSVAX B/E gp120 immunization of rhesus macaques
- Source :
- JCI Insight. 1
- Publication Year :
- 2016
- Publisher :
- American Society for Clinical Investigation, 2016.
-
Abstract
- The ALVAC prime/ALVAC + AIDSVAX B/E boost RV144 vaccine trial induced an estimated 31% efficacy in a low-risk cohort where HIV‑1 exposures were likely at mucosal surfaces. An immune correlates study demonstrated that antibodies targeting the V2 region and in a secondary analysis antibody-dependent cellular cytotoxicity (ADCC), in the presence of low envelope-specific (Env-specific) IgA, correlated with decreased risk of infection. Thus, understanding the B cell repertoires induced by this vaccine in systemic and mucosal compartments are key to understanding the potential protective mechanisms of this vaccine regimen. We immunized rhesus macaques with the ALVAC/AIDSVAX B/E gp120 vaccine regimen given in RV144, and then gave a boost 6 months later, after which the animals were necropsied. We isolated systemic and intestinal vaccine Env-specific memory B cells. Whereas Env-specific B cell clonal lineages were shared between spleen, draining inguinal, anterior pelvic, posterior pelvic, and periaortic lymph nodes, members of Env‑specific B cell clonal lineages were absent in the terminal ileum. Env‑specific antibodies were detectable in rectal fluids, suggesting that IgG antibodies present at mucosal sites were likely systemically produced and transported to intestinal mucosal sites.
- Subjects :
- 0301 basic medicine
Immunization, Secondary
HIV Infections
HIV Antibodies
HIV Envelope Protein gp120
03 medical and health sciences
0302 clinical medicine
Immunity
medicine
Animals
Memory B cell
Immunity, Mucosal
B cell
AIDS Vaccines
Antibody-dependent cell-mediated cytotoxicity
B-Lymphocytes
biology
Vaccine trial
General Medicine
Macaca mulatta
Virology
3. Good health
030104 developmental biology
medicine.anatomical_structure
Immunization
Immunoglobulin G
030220 oncology & carcinogenesis
AIDSVAX
Immunology
biology.protein
Antibody
Research Article
Subjects
Details
- ISSN :
- 23793708
- Volume :
- 1
- Database :
- OpenAIRE
- Journal :
- JCI Insight
- Accession number :
- edsair.doi.dedup.....3fcdf56204b6eea0a101540ed1185236