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Hydrolysis of Ionic Phthalic Acid Based Polyesters by Wastewater Microorganisms and Their Enzymes
- Source :
- Environmental sciencetechnology. 51(8)
- Publication Year :
- 2017
-
Abstract
- Water-soluble polyesters are used in a range of applications today and enter wastewater treatment plants after product utilization. However, little is known about extracellular enzymes and aquatic microorganisms involved in polyester biodegradation and mineralization. In this study, structurally different ionic phthalic acid based polyesters (the number-average molecular weights (Mn) 1770 to 10 000 g/mol and semi crystalline with crystallinity below 1%) were synthesized in various combinations. Typical wastewater microorganisms like Pseudomonas sp. were chosen for in-silico screening toward polyester hydrolyzing enzymes. Based on the in-silico search, a cutinase from Pseudomonas pseudoalcaligenes (PpCutA) and a putative lipase from Pseudomonas pelagia (PpelaLip) were identified. The enzymes PpCutA and PpelaLip were demonstrated to hydrolyze all structurally different polyesters. Activities on all the polyesters were also confirmed with the strains P. pseudoalcaligenes and P. pelagia. Parameters identified...
- Subjects :
- 0301 basic medicine
Cutinase
Polyesters
Phthalic Acids
010501 environmental sciences
Wastewater
01 natural sciences
03 medical and health sciences
Hydrolysis
chemistry.chemical_compound
Pseudomonas pseudoalcaligenes
Pseudomonas
Environmental Chemistry
Organic chemistry
Lipase
0105 earth and related environmental sciences
biology
Chemistry
General Chemistry
Biodegradation
biology.organism_classification
Polyester
Phthalic acid
030104 developmental biology
biology.protein
Subjects
Details
- ISSN :
- 15205851
- Volume :
- 51
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Environmental sciencetechnology
- Accession number :
- edsair.doi.dedup.....3fadb94553ddea653dbb2e36c428fb78