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Utilizing 3D Printing Technology to Merge MRI with Histology: A Protocol for Brain Sectioning
- Source :
- Journal of Visualized Experiments : JoVE
- Publication Year :
- 2016
- Publisher :
- MyJove Corporation, 2016.
-
Abstract
- Magnetic resonance imaging (MRI) allows for the delineation between normal and abnormal tissue on a macroscopic scale, sampling an entire tissue volume three-dimensionally. While MRI is an extremely sensitive tool for detecting tissue abnormalities, association of signal changes with an underlying pathological process is usually not straightforward. In the central nervous system, for example, inflammation, demyelination, axonal damage, gliosis, and neuronal death may all induce similar findings on MRI. As such, interpretation of MRI scans depends on the context, and radiological-histopathological correlation is therefore of the utmost importance. Unfortunately, traditional pathological sectioning of brain tissue is often imprecise and inconsistent, thus complicating the comparison between histology sections and MRI. This article presents novel methodology for accurately sectioning primate brain tissues and thus allowing precise matching between histology and MRI. The detailed protocol described in this article will assist investigators in applying this method, which relies on the creation of 3D printed brain slicers. Slightly modified, it can be easily implemented for brains of other species, including humans.
- Subjects :
- 0301 basic medicine
Computer science
brain
General Chemical Engineering
Central nervous system
Context (language use)
General Biochemistry, Genetics and Molecular Biology
histology
03 medical and health sciences
0302 clinical medicine
medicine
Animals
Humans
Issue 118
Sampling (medicine)
human
Process (anatomy)
marmoset
General Immunology and Microbiology
medicine.diagnostic_test
General Neuroscience
Histological Techniques
Histology
Magnetic resonance imaging
3D printing
Magnetic Resonance Imaging
030104 developmental biology
medicine.anatomical_structure
Gliosis
Printing, Three-Dimensional
medicine.symptom
Neuroscience
030217 neurology & neurosurgery
MRI
Tissue volume
Subjects
Details
- ISSN :
- 1940087X
- Database :
- OpenAIRE
- Journal :
- Journal of Visualized Experiments
- Accession number :
- edsair.doi.dedup.....3fa4dbc9766f661d26d01d0a795eb296