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In Vitro Assessment of Marine Bacillus for Use as Livestock Probiotics
- Source :
- Marine Drugs, Marine Drugs, Vol 12, Iss 5, Pp 2422-2445 (2014), Marine Drugs; Volume 12; Issue 5; Pages: 2422-2445
- Publication Year :
- 2014
- Publisher :
- MDPI AG, 2014.
-
Abstract
- Six antimicrobial-producing seaweed-derived Bacillus strains were evaluated in vitro as animal probiotics, in comparison to two Bacillus from an EU-authorized animal probiotic product. Antimicrobial activity was demonstrated on solid media against porcine Salmonella and E. coli. The marine isolates were most active against the latter, had better activity than the commercial probiotics and Bacillus pumilus WIT 588 also reduced E. coli counts in broth. All of the marine Bacillus tolerated physiological concentrations of bile, with some as tolerant as one of the probiotics. Spore counts for all isolates remained almost constant during incubation in simulated gastric and ileum juices. All of the marine Bacillus grew anaerobically and the spores of all except one isolate germinated under anaerobic conditions. All were sensitive to a panel of antibiotics and none harbored Bacillus enterotoxin genes but all, except B. pumilus WIT 588, showed some degree of β-hemolysis. However, trypan blue dye exclusion and xCELLigence assays demonstrated a lack of toxicity in comparison to two pathogens; in fact, the commercial probiotics appeared more cytotoxic than the majority of the marine Bacillus. Overall, some of the marine-derived Bacillus, in particular B. pumilus WIT 588, demonstrate potential for use as livestock probiotics.
- Subjects :
- Salmonella
Livestock
spores
medicine.drug_class
Antibiotics
Pharmaceutical Science
Bacillus
Microbial Sensitivity Tests
In Vitro Techniques
Biology
medicine.disease_cause
Hemolysis
Article
Microbiology
law.invention
Probiotic
law
xCELLigence
Drug Discovery
Cell Adhesion
medicine
Animals
Bile
Humans
Seawater
Anaerobiosis
lcsh:QH301-705.5
Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
Incubation
Spores, Bacterial
Bacillus (shape)
Bacteria
Bacillus pumilus
Probiotics
fungi
E. coli
pigs
Seaweed
Antimicrobial
biology.organism_classification
antimicrobial
Anti-Bacterial Agents
Spore
lcsh:Biology (General)
Water Microbiology
Subjects
Details
- ISSN :
- 16603397
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Marine Drugs
- Accession number :
- edsair.doi.dedup.....3f8955ab66aba5d33aee94cc0ba8102a