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Regular exercise stimulates endothelium autophagy via IL-1 signaling in ApoE deficient mice
- Source :
- FASEB journal : official publication of the Federation of American Societies for Experimental BiologyREFERENCES. 35(7)
- Publication Year :
- 2021
-
Abstract
- Regular exercise maintains arterial endothelial cell homeostasis and protects the arteries from vascular disease, such as peripheral artery disease and atherosclerosis. Autophagy, which is a cellular process that degrades misfolded or aggregate proteins and damaged organelles, plays an important role in maintaining organ and cellular homeostasis. However, it is unknown whether regular exercise stimulates autophagy in aorta endothelial cells of mice prone to atherosclerosis independently of their circulating lipid profile. Here, we observed that 16 weeks of voluntary exercise reduced high-fat diet-induced atherosclerotic plaque formation in the aortic root of ApoE deficient mice, and that this protection occurred without changes in circulating triglycerides, total cholesterol, and lipoproteins. Immunofluorescence analysis indicated that voluntary exercise increased levels of the autophagy protein LC3 in aortic endothelial cells. Interestingly, human umbilical vein endothelial cells (HUVECs) exposed to serum from voluntarily exercised mice displayed significantly increased LC3-I and LC3-II protein levels. Analysis of circulating cytokines demonstrated that voluntary exercise caused changes directly relevant to IL-1 signaling (ie, decreased interleukin-1 receptor antagonist [IL-1ra] while also increasing IL-1α). HUVECs exposed to IL-1α and IL-1β recombinant protein significantly increased LC3 mRNA expression, LC3-I and LC3-II protein levels, and autophagy flux. Collectively, these results suggest that regular exercise protects arteries from ApoE deficient mice against atherosclerosis at least in part by stimulating endothelial cell autophagy via enhanced IL-1 signaling.
- Subjects :
- 0301 basic medicine
Apolipoprotein E
Male
medicine.medical_specialty
Endothelium
Mice, Knockout, ApoE
Cellular homeostasis
Inflammation
Diet, High-Fat
Biochemistry
Umbilical vein
03 medical and health sciences
Mice
0302 clinical medicine
Internal medicine
Physical Conditioning, Animal
Genetics
medicine
Autophagy
Animals
Molecular Biology
business.industry
Atherosclerosis
Endothelial stem cell
Mice, Inbred C57BL
030104 developmental biology
medicine.anatomical_structure
Endocrinology
Endothelium, Vascular
medicine.symptom
business
030217 neurology & neurosurgery
Homeostasis
Biotechnology
Interleukin-1
Subjects
Details
- ISSN :
- 15306860
- Volume :
- 35
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- FASEB journal : official publication of the Federation of American Societies for Experimental BiologyREFERENCES
- Accession number :
- edsair.doi.dedup.....3f865d0736f56398dd455639682093de