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Impaired B cell immunity in acute myeloid leukemia patients after chemotherapy
- Source :
- Journal of Translational Medicine, Vol 15, Iss 1, Pp 1-16 (2017), Journal of Translational Medicine
- Publication Year :
- 2017
- Publisher :
- BMC, 2017.
-
Abstract
- Background Changes in adaptive immune cells after chemotherapy in adult acute myeloid leukemia (AML) may have implications for the success of immunotherapy. This study was designed to determine the functional capacity of the immune system in adult patients with AML who have completed chemotherapy and are potential candidates for immunotherapy. Methods We used the response to seasonal influenza vaccination as a surrogate for the robustness of the immune system in 10 AML patients in a complete remission post-chemotherapy and performed genetic, phenotypic, and functional characterization of adaptive immune cell subsets. Results Only 2 patients generated protective titers in response to vaccination, and a majority of patients had abnormal frequencies of transitional and memory B-cells. B-cell receptor sequencing showed a B-cell repertoire with little evidence of somatic hypermutation in most patients. Conversely, frequencies of T-cell populations were similar to those seen in healthy controls, and cytotoxic T-cells demonstrated antigen-specific activity after vaccination. Effector T-cells had increased PD-1 expression in AML patients least removed from chemotherapy. Conclusion Our results suggest that while some aspects of cellular immunity recover quickly, humoral immunity is incompletely reconstituted in the year following intensive cytotoxic chemotherapy for AML. The observed B-cell abnormalities may explain the poor response to vaccination often seen in AML patients after chemotherapy. Furthermore, the uncoupled recovery of B-cell and T-cell immunity and increased PD-1 expression shortly after chemotherapy might have implications for the success of several modalities of immunotherapy. Electronic supplementary material The online version of this article (doi:10.1186/s12967-017-1252-2) contains supplementary material, which is available to authorized users.
- Subjects :
- Male
0301 basic medicine
Cellular immunity
Time Factors
T-Lymphocytes
medicine.medical_treatment
Programmed Cell Death 1 Receptor
lcsh:Medicine
Antibodies, Viral
Medicine
B-Lymphocytes
Leukemia
Remission Induction
Vaccination
Myeloid leukemia
General Medicine
Middle Aged
Acquired immune system
Tissue Donors
Leukemia, Myeloid, Acute
Treatment Outcome
Influenza Vaccines
Female
Immunotherapy
Adult
Adaptive immunity
B-cells
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Immune system
Humans
Lymphocyte Count
Aged
Demography
business.industry
Research
T-cells
lcsh:R
Immunity
Adult Acute Myeloid Leukemia
medicine.disease
Influenza vaccination
Consolidation Chemotherapy
030104 developmental biology
Immunology
business
Immunologic Memory
Subjects
Details
- Language :
- English
- ISSN :
- 14795876
- Volume :
- 15
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of Translational Medicine
- Accession number :
- edsair.doi.dedup.....3f77ae285f1f6dbf629fd1ce12d7afc6