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Combination therapy with polydeoxyribonucleotide and proton pump inhibitor enhances therapeutic effectiveness for gastric ulcer in rats
- Source :
- Life Sciences. 203:12-19
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Aims The main action of proton pump inhibitors (PPIs) is to inhibit gastric acid secretion, and PPIs are widely used to treat gastric ulcer (GU). However, if the action of promoting gastric mucosal regeneration is added, the effectiveness of GU treatment can be enhanced. Thus, in order to improve the therapeutic effect on GU, we tried to develop combination therapy promoting regeneration in injured tissue besides suppressing gastric acid secretion. Main methods Polydeoxyribonucleotide (PDRN) was selected to evaluate tissue regeneration, and pantoprazole was chosen as one of the PPIs. GU was induced by oral administration of indomethacin once a day for 7 days. Rats in drug-administered groups were intraperitoneally injected with 100 μL normal saline, containing each drug at the indicated concentration, once a day for 14 days after inducing GU. Key findings PDRN and PPI combination therapy potently improved tissue regeneration and inhibited production of pro-inflammatory cytokines. PDRN treatment with or without PPI increased the concentration of cyclic adenosine-3,5′-monophosphate (cAMP) and the ratio of phosphorylated cAMP response element-binding protein (p-CREB) to cAMP response element-binding protein (CREB). PDRN treatment with or without PPI also increased the expressions of vascular endothelial growth factor (VEGF) and adenosine A2A receptor. Significance PDRN and PPI combination therapy showed more potent therapeutic effect on GU compared to the PDRN monotherapy or PPI monotherapy. The excellent therapeutic effect of PDRN and PPI combination therapy on GU appeared by promoting regeneration of damaged tissue as well as inhibiting gastric acid secretion.
- Subjects :
- Male
0301 basic medicine
Combination therapy
medicine.drug_class
Indomethacin
Proton-pump inhibitor
Adenosine A2A receptor
Pharmacology
General Biochemistry, Genetics and Molecular Biology
Rats, Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
Polydeoxyribonucleotides
0302 clinical medicine
Oral administration
Animals
Medicine
Stomach Ulcer
General Pharmacology, Toxicology and Pharmaceutics
Pantoprazole
business.industry
Anti-Inflammatory Agents, Non-Steroidal
Therapeutic effect
Proton Pump Inhibitors
General Medicine
Rats
Vascular endothelial growth factor
030104 developmental biology
chemistry
030220 oncology & carcinogenesis
Gastric acid
Drug Therapy, Combination
business
Signal Transduction
medicine.drug
Subjects
Details
- ISSN :
- 00243205
- Volume :
- 203
- Database :
- OpenAIRE
- Journal :
- Life Sciences
- Accession number :
- edsair.doi.dedup.....3f6469d53ce4bb09f7f4d2a638fe48be