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Adrm1 interacts with Atp6v0d2 and regulates osteoclast differentiation
- Source :
- Biochemical and Biophysical Research Communications. 390:585-590
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- Bone homeostasis is tightly regulated by matrix-producing osteoblasts and bone-resorbing osteoclasts. During osteoclast development, mononuclear preosteoclasts derived from myeloid cells fuse together to form multinucleated, giant cells. Previously, we reported that the d2 isoform of the vacuolar (H+) ATPase V0 domain (Atp6v0d2) plays an important role in osteoclast maturation and bone formation. To understand how Atp6v0d2 controls osteoclast maturation, we have performed a yeast two-hybrid screen using full-length Atp6v0d2 as the bait, and identified adhesion-regulating molecule 1 protein (Adrm1) as a potential functional partner of Atp6v0d2. The interaction between Atp6v0d2 and Adrm1 was confirmed in yeast and invivo using immunoprecipitation assays. We also show that Adrm1 is required for cell migration and osteoclast maturation.
- Subjects :
- musculoskeletal diseases
Vacuolar Proton-Translocating ATPases
Immunoprecipitation
Cellular differentiation
Biophysics
Osteoclasts
Biology
ADRM1
Biochemistry
Article
Osteoclast maturation
Mice
Multinucleate
Cell Movement
Osteoclast
Two-Hybrid System Techniques
medicine
Animals
Humans
Molecular Biology
Cells, Cultured
Membrane Glycoproteins
Cell fusion
Intracellular Signaling Peptides and Proteins
Cell Differentiation
Cell migration
Cell Biology
Molecular biology
Cell biology
medicine.anatomical_structure
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 390
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....3f5da5061220545732c15d4650fd04ff
- Full Text :
- https://doi.org/10.1016/j.bbrc.2009.10.010