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Adrm1 interacts with Atp6v0d2 and regulates osteoclast differentiation

Authors :
Hyunil Ha
Seoung Hoon Lee
Okju Yi
Taesoo Kim
Yongwon Choi
Nacksung Kim
Source :
Biochemical and Biophysical Research Communications. 390:585-590
Publication Year :
2009
Publisher :
Elsevier BV, 2009.

Abstract

Bone homeostasis is tightly regulated by matrix-producing osteoblasts and bone-resorbing osteoclasts. During osteoclast development, mononuclear preosteoclasts derived from myeloid cells fuse together to form multinucleated, giant cells. Previously, we reported that the d2 isoform of the vacuolar (H+) ATPase V0 domain (Atp6v0d2) plays an important role in osteoclast maturation and bone formation. To understand how Atp6v0d2 controls osteoclast maturation, we have performed a yeast two-hybrid screen using full-length Atp6v0d2 as the bait, and identified adhesion-regulating molecule 1 protein (Adrm1) as a potential functional partner of Atp6v0d2. The interaction between Atp6v0d2 and Adrm1 was confirmed in yeast and invivo using immunoprecipitation assays. We also show that Adrm1 is required for cell migration and osteoclast maturation.

Details

ISSN :
0006291X
Volume :
390
Database :
OpenAIRE
Journal :
Biochemical and Biophysical Research Communications
Accession number :
edsair.doi.dedup.....3f5da5061220545732c15d4650fd04ff
Full Text :
https://doi.org/10.1016/j.bbrc.2009.10.010