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ELK1-induced upregulation lncRNA LINC02381 accelerates the osteosarcoma tumorigenesis through targeting CDCA4 via sponging miR-503–5p
- Source :
- Biochemical and Biophysical Research Communications. 548:112-119
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Long noncoding RNAs (lncRNAs) have been identified as functional modulators in human tumors. The purpose of our study was to determine the expressing trend, clinical significance and functions of lncRNA LINC02381(LINC02381) in osteosarcoma. We observed that the expression of LINC02381 and cell division cycle-associated protein 4 (CDCA4) were distinctly increased in osteosarcoma specimens and cells, while miR-503–5p expression was decreased. Additionally, ETS transcription factor ELK1 (ELK1) could bind directly to the LINC02381 promoter region and activate its transcription. Clinical assays revealed that high LINC02381 was associated with advanced clinical progress and poor clinical outcome. Functionally, knockdown of LINC02381 suppressed the proliferation, migration and invasion of osteosarcoma cells. What’s more, LINC02381 could down-regulate CDCA4 via sponging miR-503–5p, and there existed a negative correlation between LINC02381 expression and miR-503–5p expression in 92 osteosarcoma samples. Rescue experiments proved the carcinogenic role of LINC02381/miR-503–5p/CDCA4 axis in osteosarcoma progression. Overall, our data illustrated how LINC02381 played an oncogenic role in osteosarcoma and might offer a novel diagnostic and prognostic biomarker and potential therapeutic target for osteosarcoma.
- Subjects :
- musculoskeletal diseases
0301 basic medicine
Carcinogenesis
Biophysics
Cell Cycle Proteins
Biology
medicine.disease_cause
Biochemistry
03 medical and health sciences
0302 clinical medicine
ELK1
Downregulation and upregulation
Transcription (biology)
Cell Line, Tumor
medicine
Humans
Gene Silencing
Neoplasm Metastasis
Molecular Biology
Cell Proliferation
ets-Domain Protein Elk-1
Osteosarcoma
Gene knockdown
Base Sequence
ETS transcription factor family
Promoter
Cell Biology
medicine.disease
Up-Regulation
Gene Expression Regulation, Neoplastic
MicroRNAs
Treatment Outcome
030104 developmental biology
030220 oncology & carcinogenesis
Cancer research
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 548
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....3f26d4b0562a88bcd08deb805b2d3442