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Expanding the application of a UV-visible reporter for transient gene expression and stable transformation in plants
- Source :
- Horticulture Research
- Publication Year :
- 2021
-
Abstract
- Green fluorescent protein (GFP) has been widely used for monitoring gene expression and protein localization in diverse organisms. However, highly sensitive imaging equipment, like fluorescence microscope, is usually required for the visualization of GFP, limitings its application to fixed locations in samples. A reporter that can be visualized in real-time regardless the shape, size and location of the target samples will increase the flexibility and efficiency of research work. Here, we report the application of a GFP-like protein, called eYGFPuv, in both transient expression and stable transformation, in two herbaceous plant species (Arabidopsisand tobacco) and two woody plant species (poplar and citrus). We observed bright fluorescence under UV light in all of the four plant species without any effects on plant growth or development. eYGFPuv was shown to be effective for imaging transient expression in leaf and root tissues. With a focus on in vitro transformation, we demonstrated that the transgenic events expressing 1xeYGFPuvcould be easily identified visually during the callus stage and the shoot stage, enabling early and efficient selection of transformants. Furthermore, whole-plant level visualization of eYGFPuv revealed its ubiquitous stability in transgenic plants. In addition, our transformation experiments showed that eYGFPuv can also be used to select transgenic plants without antibiotics. This work demonstrates the feasibility of utilizing 1xeYGFPuvin studies of gene expression and plant transformation in diverse plants.
- Subjects :
- biology
Transgene
fungi
High-throughput screening
food and beverages
Molecular engineering in plants
Plant Science
Genetically modified crops
Horticulture
biology.organism_classification
Biochemistry
Genetic vectors
Article
Cell biology
Green fluorescent protein
Transformation (genetics)
Reporter genes
Arabidopsis
Callus
Gene expression
Genetic engineering
Genetics
Fluorescence microscope
Biotechnology
Subjects
Details
- ISSN :
- 26626810
- Volume :
- 8
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Horticulture research
- Accession number :
- edsair.doi.dedup.....3f173464b3d971b117ded706b02b2e39