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Cross-Linking Furan-Modified Kisspeptin-10 to the KISS Receptor
- Source :
- ACS Chemical Biology, ACS Chemical Biology, American Chemical Society, 2017, 12 (8), pp.2191-2200. ⟨10.1021/acschembio.7b00396⟩
- Publication Year :
- 2017
-
Abstract
- Chemical cross-linking is well-established for investigating protein–protein interactions. Traditionally, photo cross-linking is used but is associated with problems of selectivity and UV toxicity in a biological context. We here describe, with live cells and under normal growth conditions, selective cross-linking of a furan-modified peptide ligand to its membrane-presented receptor with zero toxicity, high efficiency, and spatio-specificity. Furan-modified kisspeptin-10 is covalently coupled to its glycosylated membrane receptor, GPR54(KISS1R). This newly expands the applicability of furan-mediated cross-linking not only to protein–protein cross-linking but also to cross-linking in situ. Moreover, in our earlier reports on nucleic acid interstrand cross-linking, furan activation required external triggers of oxidation (via addition of N-bromo succinimide or singlet oxygen). In contrast, we here show, for multiple cell lines, the spontaneous endogenous oxidation of the furan moiety with concurrent selective cross-link formation. We propose that reactive oxygen species produced by NADPH oxidase (NOX) enzymes form the cellular source establishing furan oxidation.
- Subjects :
- 0301 basic medicine
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT]
Stereochemistry
receiver
furan
Context (language use)
gène kiss 1
Biochemistry
Models, Biological
03 medical and health sciences
chemistry.chemical_compound
Succinimide
Cell surface receptor
Cell Line, Tumor
Moiety
Humans
Amino Acid Sequence
Receptor
Furans
Kisspeptins
Singlet oxygen
kisspeptine
General Medicine
cell line
lignée cellulaire
030104 developmental biology
chemistry
furane
Nucleic acid
Biophysics
Molecular Medicine
Bioorthogonal chemistry
Reactive Oxygen Species
récepteur
Oxidation-Reduction
Receptors, Kisspeptin-1
Subjects
Details
- ISSN :
- 15548937 and 15548929
- Volume :
- 12
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- ACS chemical biology
- Accession number :
- edsair.doi.dedup.....3f1411f13e54f0a62587f2b8a91c541c
- Full Text :
- https://doi.org/10.1021/acschembio.7b00396⟩