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Determination of the changes in the gastric fluid endurance of O157 and non-O157 Shiga toxin-producing Escherichia coli during storage of experimentally produced beef frankfurter
- Source :
- J Food Sci Technol
- Publication Year :
- 2020
- Publisher :
- Springer India, 2020.
-
Abstract
- Resistance of Shiga toxin-producing Escherichia coli (STEC) O157:H7 and serogroups O103, O26 and O145 to synthetic gastric fluid (SGF, pH 1.5) were investigated during frankfurter storage. Pathogens were inoculated (5 ± 1 log(10 )cfu g(−1)) on frankfurters and frankfurters were stored at 4 °C for 75 days in vacuum packages. Population changes of the competitive flora and STEC, changes in the pH of the frankfurters and resistance of STEC to SGF were monitored on days 0, 15, 30, 45, 60 and 75 of frankfurter storage. Direct synthetic gastric fluid (DSGF) challenges were also conducted to assess pathogen resistance without being effected by frankfurters, by inoculating pathogen cultures directly into SGF. Results showed that acid resistance of O145 and O26 was stronger than that of O103 and O157 during frankfurter storage. Resistance of O103 to SGF was better than that of O157 during frankfurter storage but, was similar to that of O157 during DSGF challenges. Results indicate that acid resistance of some strains of STEC pathogens might differentiate during storage of frankfurters. Different resistance capabilities to SGF were observed in the STEC strains when inoculated and stored on frankfurters than directly inoculated in the SGF.
- Subjects :
- 0303 health sciences
education.field_of_study
Gastric fluid
030306 microbiology
Chemistry
Pathogen resistance
Population
0402 animal and dairy science
Acid resistance
04 agricultural and veterinary sciences
bacterial infections and mycoses
medicine.disease_cause
040201 dairy & animal science
Non o157
03 medical and health sciences
fluids and secretions
medicine
Original Article
Food science
education
Escherichia coli
Pathogen
Shiga toxin-producing Escherichia coli
Food Science
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- J Food Sci Technol
- Accession number :
- edsair.doi.dedup.....3ef5d64d5c86f215633385a5b596d92b