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AutoTube: a novel software for the automated morphometric analysis of vascular networks in tissues
- Source :
- Angiogenesis, Angiogenesis, 22 (2)
- Publication Year :
- 2019
- Publisher :
- ETH Zurich, 2019.
-
Abstract
- Due to their involvement in many physiologic and pathologic processes, there is a great interest in identifying new molecular pathways that mediate the formation and function of blood and lymphatic vessels. Vascular research increasingly involves the image-based analysis and quantification of vessel networks in tissue whole-mounts or of tube-like structures formed by cultured endothelial cells in vitro. While both types of experiments deliver important mechanistic insights into (lymph)angiogenic processes, the manual analysis and quantification of such experiments are typically labour-intensive and affected by inter-experimenter variability. To bypass these problems, we developed AutoTube, a new software that quantifies parameters like the area covered by vessels, vessel width, skeleton length and branching or crossing points of vascular networks in tissues and in in vitro assays. AutoTube is freely downloadable, comprises an intuitive graphical user interface and helps to perform otherwise highly time-consuming image analyses in a rapid, automated and reproducible manner. By analysing lymphatic and blood vascular networks in whole-mounts prepared from different tissues or from gene-targeted mice with known vascular abnormalities, we demonstrate the ability of AutoTube to determine vascular parameters in close agreement to the manual analyses and to identify statistically significant differences in vascular morphology in tissues and in vascular networks formed in in vitro assays.<br />Angiogenesis, 22 (2)
- Subjects :
- 0301 basic medicine
Cancer Research
Lymphatic vessels
Blood vessels
Quantification
Morphometric analysis
Whole-mounts
Tube formation
Physiology
Angiogenesis
Clinical Biochemistry
Neovascularization, Physiologic
Cell Count
Cell Communication
Biology
03 medical and health sciences
Mice
0302 clinical medicine
Software
Image Processing, Computer-Assisted
Animals
Humans
Lymphangiogenesis
Cells, Cultured
Cell Size
Whole mount
Mice, Knockout
Original Paper
business.industry
In vitro toxicology
Endothelial Cells
Mice, Inbred C57BL
030104 developmental biology
Lymphatic system
Vascular morphology
030220 oncology & carcinogenesis
Microvessels
business
Biomedical engineering
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Angiogenesis, Angiogenesis, 22 (2)
- Accession number :
- edsair.doi.dedup.....3ef2056b9fe59070ec4f9d88380e5718
- Full Text :
- https://doi.org/10.3929/ethz-b-000315996