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T cell epitope immunotherapy ameliorates allergic responses in a murine model of shrimp allergy
- Source :
- Clinical & Experimental Allergy. 46:491-503
- Publication Year :
- 2016
- Publisher :
- Wiley, 2016.
-
Abstract
- BACKGROUND Shellfish allergy is one of the most common food hypersensitivities worldwide but allergen-specific immunotherapy for shellfish allergy is not yet available. We believe that T cell peptide-based immunotherapy holds the potential for modulating allergic responses without IgE cross-linking. OBJECTIVE We sought to identify the immunodominant T cell epitopes of tropomyosin, the major shrimp allergen of Metapenaeus ensis (Met e 1), and to evaluate their therapeutic effects in a Balb/c mouse model of Met e 1 hypersensitivity. METHODS T cell epitopes of Met e 1 were first identified based on the proliferation and cytokine responses of splenocytes isolated from Met e 1-sensitized Balb/c mice upon stimulation by 18 synthetic peptides that span the full-length Met e 1. The immunodominant T cell peptides identified were then fed orally to Met e 1-sensitized Balb/c mice twice a week for four weeks. Allergic responses, serological antibody levels, intestinal histology and systemic and local cytokine profiles were compared between the treated and the untreated groups. RESULTS Six major Met e 1 T cell epitopes were identified. Mice treated with the T cell epitope peptide mixture demonstrated an amelioration of systemic allergic symptoms and a significant reduction in Th2-associated antibody and cytokine responses. These benefits were accompanied by a shift to a balanced Th1/Th2 response, induction of IgG2a antibodies possessing in vitro and in vivo blocking activities and the induction of regulatory T cell responses. CONCLUSIONS AND CLINICAL RELEVANCE T cell epitope-based oral immunotherapy is effective in reducing allergic responses towards shrimp tropomyosin. This is a novel strategy for clinical management of shellfish allergy and is a model for mechanistic studies of oral immunotherapy.
- Subjects :
- Allergy
Regulatory T cell
medicine.medical_treatment
T cell
Immunology
Epitopes, T-Lymphocyte
Biology
Immunoglobulin E
Epitope
Mice
03 medical and health sciences
0302 clinical medicine
Penaeidae
Antibody Specificity
medicine
Animals
Humans
Immunology and Allergy
Amino Acid Sequence
Th1-Th2 Balance
Shellfish allergy
Proteins
Immunotherapy
Allergens
medicine.disease
Disease Models, Animal
medicine.anatomical_structure
Epitope mapping
030228 respiratory system
Desensitization, Immunologic
Immunoglobulin G
biology.protein
Cytokines
Immunization
Peptides
Biomarkers
Epitope Mapping
Food Hypersensitivity
030215 immunology
Subjects
Details
- ISSN :
- 09547894
- Volume :
- 46
- Database :
- OpenAIRE
- Journal :
- Clinical & Experimental Allergy
- Accession number :
- edsair.doi.dedup.....3ee483e02717aaccba7c0fdc5b9ae3d2