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Force-Controlled Formation of Dynamic Nanopores for Single-Biomolecule Sensing and Single-Cell Secretomics
- Source :
- ACS Nano, 14 (10)
- Publication Year :
- 2020
- Publisher :
- American Chemical Society, 2020.
-
Abstract
- Nanopore sensing of single nucleotides has emerged as a promising single-molecule technology for DNA sequencing and proteomics. Despite the conceptual simplicity of nanopores, adoption of this technology for practical applications has been limited by a lack of pore size adjustability and an inability to perform long-term recordings in complex solutions. Here we introduce a method for fast and precise on-demand formation of a nanopore with controllable size between 2 and 20 nm through force-controlled adjustment of the nanospace formed between the opening of a microfluidic device (made of silicon nitride) and a soft polymeric substrate. The introduced nanopore system enables stable measurements at arbitrary locations. By accurately positioning the nanopore in the proximity of single neurons and continuously recording single-molecule translations over several hours, we have demonstrated this is a powerful approach for single-cell proteomics and secretomics.<br />ACS Nano, 14 (10)<br />ISSN:1936-0851<br />ISSN:1936-086X
Details
- Language :
- English
- ISSN :
- 19360851 and 1936086X
- Database :
- OpenAIRE
- Journal :
- ACS Nano, 14 (10)
- Accession number :
- edsair.doi.dedup.....3ed1e40a3c078e83cabdc965564349d0