Back to Search
Start Over
miR-205 is a critical regulator of lacrimal gland development
- Source :
- Dev Biol
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- The tear film protects the terrestrial animal’s ocular surface and the lacrimal gland provides important aqueous secretions necessary for its maintenance. Despite the importance of the lacrimal gland in ocular health, molecular aspects of its development remain poorly understood. We have identified a noncoding RNA (miR-205) as an important gene for lacrimal gland development. Mice lacking miR-205 fail to properly develop lacrimal glands, establishing this noncoding RNA as a key regulator of lacrimal gland development. Specifically, more than half of knockout lacrimal glands never initiated, suggesting a critical role of miR-205 at the earliest stages of lacrimal gland development. RNA-seq analysis uncovered several up-regulated miR-205 targets that may interfere with signaling to impair lacrimal gland initiation. Supporting this data, combinatorial epistatic deletion of Fgf10, the driver of lacrimal gland initiation, and miR-205 in mice exacerbates the lacrimal gland phenotype. We develop a molecular rheostat model where miR-205 modulates signaling pathways related to Fgf10 in order to regulate glandular development. These data show that a single microRNA is a key regulator for early lacrimal gland development in mice and highlights the important role of microRNAs during organogenesis.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Organogenesis
Regulator
Fluorescent Antibody Technique
Mice, Transgenic
Lacrimal gland
Biology
Article
03 medical and health sciences
0302 clinical medicine
stomatognathic system
Internal medicine
microRNA
medicine
Animals
Molecular Biology
Mice, Knockout
FGF10
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, RNA
Gene Expression Profiling
Lacrimal Apparatus
Gene Expression Regulation, Developmental
Cell Biology
Non-coding RNA
Phenotype
Cell biology
Mice, Inbred C57BL
MicroRNAs
030104 developmental biology
Endocrinology
medicine.anatomical_structure
Signal transduction
Fibroblast Growth Factor 10
030217 neurology & neurosurgery
Signal Transduction
Developmental Biology
Subjects
Details
- ISSN :
- 00121606
- Volume :
- 427
- Database :
- OpenAIRE
- Journal :
- Developmental Biology
- Accession number :
- edsair.doi.dedup.....3eb6be1b50c09bea8525685064af5b45