A DNA fingerprinting method for individual characterization ofToxoplasma gondii strains: combination with isoenzymatic characters for determination of linkage groups

The genetic polymorphism of Toxoplasma gondii was evaluated for 14 strains by isoenzyme and DNA analysis. The 14 strains belonged to 5 different zymodemes defined by the variable patterns of 6 enzyme systems. A restriction-fragment-length polymorphism analysis was carried out with two endonucleases (Sal I and Pst I) and two repetitive probes (TGR1E and TGR6). This kind of repetitive probe allowed an individual identification of strain, with 13 schizodemes being observed among 14 strains. Only two strains were found to be totally identical when DNA and isoenzyme characters were considered. The numerical taxonomy methods applied to the results obtained for both types of characters allowed determination of linkage groups. Strain clustering obtained by numerical analysis of DNA characters alone is similar to the clustering obtained by analysis of isoenzyme and DNA characters together. A relationship was observed between the defined groups and virulence in Swiss mice.