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Overlapping genes at the DNA primase locus of the large plasmid CoII
- Source :
- Nucleic Acids Research. 10:855-869
- Publication Year :
- 1982
- Publisher :
- Oxford University Press (OUP), 1982.
-
Abstract
- The sog gene of the large plasmid ColIdrd-1 has previously been shown to encode a DNA primase and a smaller antigenically related polypeptide. Genesis of these two products has been examined using Sog+ recombinant plasmids. Effects of amber mutations, isolated after in vitro mutagenesis, and deletions into or within sog suggest that the smaller polypeptide is a separate translation product which is encoded by DNA specifying the C-terminal region of the larger protein. Under control of the lac promotor, synthesis of both polypeptides is reduced when transcription is repressed. These findings imply that transcription of sog yields a single transcript which is translated from two initiation sites.
- Subjects :
- Chloramphenicol O-Acetyltransferase
DNA Replication
DNA, Recombinant
DNA Primase
Biology
law.invention
chemistry.chemical_compound
Plasmid
Acetyltransferases
Transcription (biology)
law
Escherichia coli
Genetics
Gene
DNA replication
RNA Nucleotidyltransferases
Promoter
DNA Restriction Enzymes
Molecular biology
chemistry
Protein Biosynthesis
Mutation
Recombinant DNA
Primase
DNA
Plasmids
Research Article
Subjects
Details
- ISSN :
- 13624962 and 03051048
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Nucleic Acids Research
- Accession number :
- edsair.doi.dedup.....3eaa1fac5543fb1121e8d67c68962f27