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The Colletotrichum higginsianum secreted effector protein ChEC91 induces plant cell death

Authors :
Jochen Kleemann
Kenji Ogura
Natsuki Omura
Shota Nakajima
Hiroyuki Takahara
Sho Yamaguchi
Satomi Tanaka
Richard J. O'Connell
Kasumi Otoku
ISHIKAWA PREFECTURAL UNIVERSITY JPN
Partenaires IRSTEA
Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)
Max Planck Inst Plant Breeding Res, Dept Plant Breeding & Genet, Cologne, Germany
Partenaires INRAE
BIOlogie et GEstion des Risques en agriculture (BIOGER)
AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI)JP25850029
Mitani Foundation for Research and Development
Source :
Journal of General Plant Pathology, Journal of General Plant Pathology, Springer Verlag, 2021
Publication Year :
2021
Publisher :
Springer Science and Business Media LLC, 2021.

Abstract

International audience; ChEC91, a novel cell death-inducing effector protein from the fungal pathogen Colletotrichum higginsianum, causal agent of crucifer anthracnose disease, is described. Both transient expression of ChEC91 and infiltration of purified recombinant protein induced necrotic lesions in Nicotiana benthamiana leaves. The recombinant protein also induced electrolyte leakage and callose deposition in Arabidopsis thaliana leaf tissue and the expression of defence marker genes. Moreover, fungal mutants constitutively over-expressing ChEC91 in C. higginsianum were impaired in appressorial penetration on Brassica rapa cotyledons. These results suggest that inappropriate expression of ChEC91 might negatively affect the early stage of C. higginsianum infection by inducing plant defence responses. Protein domain deletion analysis showed that the C-terminal region of ChEC91 was necessary, but not sufficient, for activity in N. benthamiana. Homologous effector proteins cloned from C. gloeosporioides, Fusarium graminearum, and Pyricularia oryzae differed in their cell death-inducing activity, which appeared related to sequence variations in the C-terminal region of these proteins. Moreover, this region contained amino acid residues that were well conserved within Colletotrichum species. These results suggest that the amino acid residues in the C-terminal region may be important for inducing cell death in plants.

Details

ISSN :
1610739X and 13452630
Volume :
87
Database :
OpenAIRE
Journal :
Journal of General Plant Pathology
Accession number :
edsair.doi.dedup.....3e99ee82934b90d420278a7f9cbbb9c8
Full Text :
https://doi.org/10.1007/s10327-021-01028-3