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The Colletotrichum higginsianum secreted effector protein ChEC91 induces plant cell death
- Source :
- Journal of General Plant Pathology, Journal of General Plant Pathology, Springer Verlag, 2021
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- International audience; ChEC91, a novel cell death-inducing effector protein from the fungal pathogen Colletotrichum higginsianum, causal agent of crucifer anthracnose disease, is described. Both transient expression of ChEC91 and infiltration of purified recombinant protein induced necrotic lesions in Nicotiana benthamiana leaves. The recombinant protein also induced electrolyte leakage and callose deposition in Arabidopsis thaliana leaf tissue and the expression of defence marker genes. Moreover, fungal mutants constitutively over-expressing ChEC91 in C. higginsianum were impaired in appressorial penetration on Brassica rapa cotyledons. These results suggest that inappropriate expression of ChEC91 might negatively affect the early stage of C. higginsianum infection by inducing plant defence responses. Protein domain deletion analysis showed that the C-terminal region of ChEC91 was necessary, but not sufficient, for activity in N. benthamiana. Homologous effector proteins cloned from C. gloeosporioides, Fusarium graminearum, and Pyricularia oryzae differed in their cell death-inducing activity, which appeared related to sequence variations in the C-terminal region of these proteins. Moreover, this region contained amino acid residues that were well conserved within Colletotrichum species. These results suggest that the amino acid residues in the C-terminal region may be important for inducing cell death in plants.
- Subjects :
- 0106 biological sciences
[SDV]Life Sciences [q-bio]
Protein domain
Mutant
Nicotiana benthamiana
Plant Science
Biology
01 natural sciences
Microbiology
03 medical and health sciences
chemistry.chemical_compound
Cell death-inducing proteinColletotrichum higginsianumEffectorRecombinant proteinTransient expression
Arabidopsis thaliana
Gene
Colletotrichum higginsianum
030304 developmental biology
0303 health sciences
Effector
fungi
Callose
food and beverages
biology.organism_classification
chemistry
[SDE]Environmental Sciences
Agronomy and Crop Science
010606 plant biology & botany
Subjects
Details
- ISSN :
- 1610739X and 13452630
- Volume :
- 87
- Database :
- OpenAIRE
- Journal :
- Journal of General Plant Pathology
- Accession number :
- edsair.doi.dedup.....3e99ee82934b90d420278a7f9cbbb9c8
- Full Text :
- https://doi.org/10.1007/s10327-021-01028-3