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Detection of Escherichia coli O157:H7 and Salmonella enterica serotype Typhimurium based on cell elongation induced by beta-lactam antibiotics
- Source :
- The Analyst. 144(15)
- Publication Year :
- 2019
-
Abstract
- Pathogenic bacteria such as Shiga toxigenic Escherichia coli and Salmonella can cause severe food-borne diseases. Rapid and sensitive detection of these foodborne pathogens is essential to ensure food safety. In this study, a novel method based on cell elongation induced by beta-lactam antibiotics for direct microscopic counting of Gram-negative bacteria was established. Combined with the sample preparation steps of membrane filtration and magnetic separation, the detection of E. coli O157:H7 and Salmonella enterica serotype Typhimurium was achieved by direct optical microscopic counting of the number of elongated bacteria. The limit of detection of E. coli O157:H7 and S. typhimurium could reach 20 CFU mL-1. The recovery tests for E. coli O157:H7 and S. typhimurium in water and milk samples showed acceptable recovery values between 93.6% and 106.2%. This method is sensitive, cost effective, and rapid (
- Subjects :
- Serotype
Salmonella typhimurium
Salmonella
medicine.drug_class
Antibiotics
medicine.disease_cause
Escherichia coli O157
Biochemistry
Analytical Chemistry
Microbiology
Aztreonam
Limit of Detection
Electrochemistry
medicine
Environmental Chemistry
Animals
Escherichia coli
Spectroscopy
biology
business.industry
Chemistry
Immunomagnetic Separation
Magnetic Phenomena
Antibodies, Monoclonal
Pathogenic bacteria
biology.organism_classification
Food safety
Bacterial Load
Anti-Bacterial Agents
Milk
Salmonella enterica
business
Water Microbiology
Bacteria
Subjects
Details
- ISSN :
- 13645528
- Volume :
- 144
- Issue :
- 15
- Database :
- OpenAIRE
- Journal :
- The Analyst
- Accession number :
- edsair.doi.dedup.....3e8330e5132814f90899b7ddcc45802a