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Fourier processing of liquid chromatograms using flow radioactive detection
- Source :
- Analytical Biochemistry, Analytical Biochemistry, Elsevier Masson, 1995, 224 (1), pp.354-63. ⟨10.1006/abio.1995.1051⟩, Analytical Biochemistry, Elsevier Masson, 1995, 224, pp.354-363
- Publication Year :
- 1995
- Publisher :
- HAL CCSD, 1995.
-
Abstract
- International audience; Flow radioactivity counters coupled to liquid chromatography devices cause a systematic bias to the separation by broadening peaks within the radiochromatogram. Such signal smearing may be evaluated on standardization runs with a single peak, using the ratio between Fourier transforms of whole chromatographic data for the measured radioactivity time series (radioactivity channel) and for the concentration time series (optical density channel). This ratio constitutes a kernel suitable to perform the deconvolution of any radiochromatogram performed under similar conditions. Through deconvolution, the signal smearing is removed, reverting to peaks with the same geometry as in the concentration chromatogram: same retention time, peak width, and shape. The deconvolution method in processing radiochromatograms allows an easier interpretation and gives more reliable radioactivity quantification (improved linearity of the measured response). Fourier transformation of the radiochromatogram also allows the removal of transitory events (noise) through the correlation procedure. This method may provide substantial gain in sensitivity, depending upon the residence time of the sample in the counting cell.
- Subjects :
- [SDV.BIO]Life Sciences [q-bio]/Biotechnology
Channel (digital image)
Biophysics
Analytical chemistry
Biochemistry
Signal
symbols.namesake
Sensitivity (control systems)
Radiometry
Molecular Biology
Residence time (statistics)
Chromatography
Liquid
Fourier Analysis
Chemistry
Noise (signal processing)
MESH: Radiometry
Linearity
Cell Biology
ACTIVITE SPECIFIQUE
[SDV.BIO] Life Sciences [q-bio]/Biotechnology
Fourier transform
symbols
Deconvolution
MESH: Chromatography, Liquid
MESH: Fourier Analysis
Chromatography, Liquid
[SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis
Subjects
Details
- Language :
- English
- ISSN :
- 00032697 and 10960309
- Database :
- OpenAIRE
- Journal :
- Analytical Biochemistry, Analytical Biochemistry, Elsevier Masson, 1995, 224 (1), pp.354-63. ⟨10.1006/abio.1995.1051⟩, Analytical Biochemistry, Elsevier Masson, 1995, 224, pp.354-363
- Accession number :
- edsair.doi.dedup.....3e762b41675214f08af4ffd216078f41