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2-DE proteomic analysis of HSP70 in mollusc Chamelea gallina
- Source :
- Fishshellfish immunology. 30(2)
- Publication Year :
- 2010
-
Abstract
- Bidimensional electrophoresis (2-DE) protocols were adapted on Chamelea gallina digestive glands studies by the analysis of Heat Shock Proteins (HSP) compared with monodimensional electrophoresis (1-DE) results. Because polycyclic aromatic hydrocarbons (PAH) act on HSPs, C. gallina specimens were exposed to 0.5 mg/L of benzo[a]pyrene (B[a]P) for 24 h, 7 and 12 days. Immunoblotting after 1-DE showed a single band of 70 kDa significantly induced after 7 days of B[a]P exposure. After 2-DE, eight major high-resolved spots between 17 and 98 kDa were revealed. Three spots fell within the range of 62–98 kDa and of 5–6 pI, parameters which could include HSP70. Two spots of 77 and 72 kDa, obtained after 2-DE immunoblotting, could correspond to constitutive HSC70 and to inducible HSP70 forms respectively. Changes observed in inducible and in constitutive forms might be related to an adaptation to stress and to a normal protein synthesis capability, respectively. Employment of 2-DE and relationship between HSP70 and HSC70 may be useful to clarify their role in molluscs subjected to stress events.
- Subjects :
- Proteomics
HSC70
Aquatic Science
chemistry.chemical_compound
Heat shock protein
Benzo(a)pyrene
Environmental Chemistry
Animals
Electrophoresis, Gel, Two-Dimensional
HSP70 Heat-Shock Proteins
Normal protein
HSP70
Spots
biology
General Medicine
Single band
2-DE
biology.organism_classification
Hsp70
Electrophoresis
Biochemistry
chemistry
Gene Expression Regulation
Mollusca
Pyrene
Chamelea gallina
CHAMELEA GALLINA
Digestive System
Water Pollutants, Chemical
Subjects
Details
- ISSN :
- 10959947
- Volume :
- 30
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Fishshellfish immunology
- Accession number :
- edsair.doi.dedup.....3e6df024a37808cf49018ce373453430