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The identification and determination of toxin genes of Vibrio strains causing hemorrhagic disease on red drum (Sciaenops ocellatus) using PCR
- Source :
- AMB Express, AMB Express, Vol 11, Iss 1, Pp 1-8 (2021)
- Publication Year :
- 2021
- Publisher :
- Springer Berlin Heidelberg, 2021.
-
Abstract
- Data were collected from 30 strains of Vibrio and sampled on different organs (brain, hemorrhagic site and digestive tract) of Sciaenops ocellatus infection. The results showed that the nucleotide sequences 16S rRNA region are highly similar to those of V. alginolyticus, V. azureus, V. fluvialis, V. natriengens and V. orientalis, which were published on Genbank and other, ranging from 98.05 to 100%. The digestive tract has the most common Vibrio strains (V. alginolyticus [16] V. azureus [7] and V. fluvialis). Thereout, 25 of 30 strains of Vibrio contained 1 to 3 toxin genes, except V. parahaemolyticus. Six parameters were used to measure the DNA polymorphism of 33 homologous DNA sequences in this Vibrio bacteria population. The results indicated that number of separate polymorphic sites (S), total number of mutant sites (Eta), number of haplotype (h), haplotype diversity (Hd), average number of nucleotide differences (k), nucleotide diversity (Pi) were 98 (S), 103 (Eta), 9 (h), 0.887 ± 0.032 (Hd), 25.789 (k) and 17.980 × 10–3 ± 0.003 (Pi), respectively (P Vibrio on 3 organs of fish (S. ocellatus) hemorrhagic are V. azureus (27,67%), V. alginolyticus (50%), V. orientalis (6,67%) and V. fluvialis (16,67%). Through this result, we found that the diversity of Vibrio species that appeared on the red drum was used in the 16S rRNA region and the presence of toxin genes in these Vibrio species.
- Subjects :
- TLH
lcsh:Biotechnology
TRH
Population
Biophysics
lcsh:QR1-502
ToxR
TDH
medicine.disease_cause
Applied Microbiology and Biotechnology
lcsh:Microbiology
Microbiology
Nucleotide diversity
03 medical and health sciences
lcsh:TP248.13-248.65
medicine
education
030304 developmental biology
Vibrio
0303 health sciences
education.field_of_study
biology
Phylogenetic tree
Toxin
Haplotype
04 agricultural and veterinary sciences
biology.organism_classification
16S ribosomal RNA
Sciaenops ocellatus
GenBank
040102 fisheries
0401 agriculture, forestry, and fisheries
Toxin gene
Original Article
Subjects
Details
- Language :
- English
- ISSN :
- 21910855
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- AMB Express
- Accession number :
- edsair.doi.dedup.....3e5ae7bc922bb7e10d6e5e7563734f06