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The isolation and mapping of a novel hydroxycinnamoyltransferase in the globe artichoke chlorogenic acid pathway
- Source :
- BMC Plant Biology, BMC Plant Biology, Vol 9, Iss 1, p 30 (2009), BMC Plant Biology, BioMed Central, 2009, 9 (1), pp.30. ⟨10.1186/1471-2229-9-30⟩, BMC Plant Biology (9), 1-13. (2009)
- Publication Year :
- 2008
-
Abstract
- Background The leaves of globe artichoke and cultivated cardoon (Cynara cardunculus L.) have significant pharmaceutical properties, which mainly result from their high content of polyphenolic compounds such as monocaffeoylquinic and dicaffeoylquinic acid (DCQ), and a range of flavonoid compounds. Results Hydroxycinnamoyl-CoA:quinate hydroxycinnamoyltransferase (HQT) encoding genes have been isolated from both globe artichoke and cultivated cardoon (GenBank accessions DQ915589 and DQ915590, respectively) using CODEHOP and PCR-RACE. A phylogenetic analysis revealed that their sequences belong to one of the major acyltransferase groups (anthranilate N-hydroxycinnamoyl/benzoyltransferase). The heterologous expression of globe artichoke HQT in E. coli showed that this enzyme can catalyze the esterification of quinic acid with caffeoyl-CoA or p-coumaroyl-CoA to generate, respectively, chlorogenic acid (CGA) and p-coumaroyl quinate. Real time PCR experiments demonstrated an increase in the expression level of HQT in UV-C treated leaves, and established a correlation between the synthesis of phenolic acids and protection against damage due to abiotic stress. The HQT gene, together with a gene encoding hydroxycinnamoyl-CoA:shikimate/quinate hydroxycinnamoyltransferase (HCT) previously isolated from globe artichoke, have been incorporated within the developing globe artichoke linkage maps. Conclusion A novel acyltransferase involved in the biosynthesis of CGA in globe artichoke has been isolated, characterized and mapped. This is a good basis for our effort to understand the genetic basis of phenylpropanoid (PP) biosynthesis in C. cardunculus.
- Subjects :
- 0106 biological sciences
Flavonoid
Shikimic Acid
Plant Science
01 natural sciences
chemistry.chemical_compound
Gene Expression Regulation, Plant
lcsh:Botany
Cynara scolymus
feuille végétal
Cloning, Molecular
cartographie chromosomique
Globe Artichoke
Plant Proteins
chemistry.chemical_classification
0303 health sciences
Phenylpropanoid
Chromosome Mapping
Quinic acid
[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]
lcsh:QK1-989
Acyl Donor
acide chlorogénique
RNA, Plant
Acyltransferase
cynara cardunculus
Chlorogenic Acid
isolation
Research Article
DNA, Complementary
purification
expression génique
Molecular Sequence Data
Quinic Acid
Biology
Polymorphism, Single Nucleotide
03 medical and health sciences
Chlorogenic acid
Botany
Amino Acid Sequence
030304 developmental biology
Cynara
Sequence Analysis, DNA
biology.organism_classification
acyltransférase
enzyme
chemistry
plante légumière
Heterologous expression
Sequence Alignment
Acyltransferases
010606 plant biology & botany
Subjects
Details
- ISSN :
- 14712229
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- BMC plant biology
- Accession number :
- edsair.doi.dedup.....3e420ef0b54e4770526c1786fd97bbbe