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Activation by phospholipids of particulate mitochondrial ATPase from rat liver
- Source :
- Biochimica et Biophysica Acta (BBA) - Biomembranes. 274:528-535
- Publication Year :
- 1972
- Publisher :
- Elsevier BV, 1972.
-
Abstract
- 1. 1. The activation by phospholipids of particulate mitochondrial ATPase from rat liver was studied on a preparation extracted from submitochondrial particles with cholate. 2. 2. Lysophosphatidylcholine and the highly acidic phospholipids diphosphatidylglycerol, phosphatidylinositol and phosphatidylserine stimulated the ATPase at low concentration (50–60 μg/mg protein); phosphatidylethanolamine was less effective whereas phosphatidylcholine exhibited the lowest activity. 3. 3. The saturation curves of phospholipids acting at low concentration showed in most cases a deviation from normal Michaelis-Menten kinetics. This devation was absent or less manifest with phosphatidylethanolamine and phosphatidylcholine. Competition between phospholipids and oligomycin was seen with acidic phospholipids or mixtures of neutral and acidic phospholipids. 4. 4. Similarly to oligomycin, dicyclohexylcarbodiimide, tributyltin and chlorpromazine competitively inhibited the activation produced by phosphatidylserine, phosphatidylinositol and diphosphatidylglycerol. 5. 5. It is concluded that both the hydrophobic-hydrophilic balance and the negative charge of phospholipids are important in the interaction between phospholipids and protein at the level of particulate mitochondrial ATPase. The competition between phospholipids and inhibitors of mitochondrial ATPase suggests that the activation induced by phospholipids is involved in the inhibitory effect.
- Subjects :
- Oligomycin
Chlorpromazine
ATPase
Biophysics
Mitochondria, Liver
Phosphatidylinositols
Biochemistry
Bile Acids and Salts
chemistry.chemical_compound
Cyclohexanes
Phosphatidylcholine
Organometallic Compounds
Animals
Submitochondrial particle
Phosphatidylinositol
Phospholipids
Adenosine Triphosphatases
Phosphatidylethanolamine
Membranes
biology
Phosphatidylethanolamines
Lysophosphatidylcholines
Cell Biology
Phosphatidylserine
Rats
Enzyme Activation
Kinetics
Lysophosphatidylcholine
chemistry
Tin
Phosphatidylcholines
biology.protein
Oligomycins
lipids (amino acids, peptides, and proteins)
Imines
Subjects
Details
- ISSN :
- 00052736
- Volume :
- 274
- Database :
- OpenAIRE
- Journal :
- Biochimica et Biophysica Acta (BBA) - Biomembranes
- Accession number :
- edsair.doi.dedup.....3e41aa93923a7b19de75d78b6e41aa0d