Back to Search
Start Over
Immunogenomics guided design of immunomodulatory multi-epitope subunit vaccine against the SARS-CoV-2 new variants, and its validation through in silico cloning and immune simulation
- Source :
- Computers in Biology and Medicine
- Publication Year :
- 2021
-
Abstract
- Reports of the novel and more contagious strains of SARS-CoV-2 originating in different countries have further aggravated the pandemic situation. The recent substitutions in spike protein may be critical for the virus to evade the host's immune system and therapeutics that have already been developed. Thus, this study has employed an immunoinformatics pipeline to target the spike protein of this novel strain to construct an immunogenic epitope (CTL, HTL, and B cell) vaccine against the new variant. Our investigation revealed that 12 different epitopes imparted a critical role in immune response induction. This was validated by an exploration of physiochemical properties and experimental feasibility. In silico and host immune simulation confirmed the expression and induction of both primary and secondary immune factors such as IL, cytokines, and antibodies. The current study warrants further lab experiments to demonstrate its efficacy and safety.<br />Graphical abstract Image 1
- Subjects :
- 0301 basic medicine
Immune simulation
In silico
Epitopes, T-Lymphocyte
Health Informatics
Computational biology
New variant
Article
Epitope
Virus
03 medical and health sciences
0302 clinical medicine
Immune system
Immunity
Humans
Vaccine designing
Computer Simulation
Cloning, Molecular
biology
SARS-CoV-2
Viral Vaccine
COVID-19
Viral Vaccines
Computer Science Applications
Molecular Docking Simulation
CTL
030104 developmental biology
Spike Glycoprotein, Coronavirus
Vaccines, Subunit
biology.protein
Epitopes, B-Lymphocyte
Antibody
In silico cloning
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 18790534
- Volume :
- 133
- Database :
- OpenAIRE
- Journal :
- Computers in biology and medicine
- Accession number :
- edsair.doi.dedup.....3e244527780bb7e019e6fc89da0ff4be