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Licochalcone A Induces Cholangiocarcinoma Cell Death Via Suppression of Nrf2 and NF-κB Signaling Pathways
- Source :
- Asian Pacific Journal of Cancer Prevention. 23:115-123
- Publication Year :
- 2022
- Publisher :
- EpiSmart Science Vector Ltd, 2022.
-
Abstract
- To investigate the anti-tumor effect of licochalcone A (LCA) on proliferation and migration in cholangiocarcinoma (CCA) cells and to elucidate their underlying mechanisms.Human CCA cells, KKU-100, KKU-213, KKU-214, KKU-156, and KKU-452 were used to study effect of LCA on proliferation and migration by a cytotoxicity assay, wound healing assay. Reactive oxygen species levels were evaluated using DHE-fluorescent probes. Proteins associated with cancer survival and progression were analyzed by immune blotting assay.LCA suppressed proliferation and induced cell death in CCA cells including KKU-100, KKU-213, KKU-214, KKU-156, and KKU-452. The CCAs cells were suppressed in association with LCA-induced accumulation of intracellular reactive oxygen species (ROS). Increased formation of ROS was causally related with suppression of Nrf2 and its down-stream antioxidant and cytoprotective enzymes. These effects may lead to the expression of Bax and release of cytochrome c and ensuring cell death. Interestingly, LCA could also inhibit cell migration and cell cycle arrest at low concentrations. These effects were associated with down-regulation of NF-kB, STAT3 and their down-stream proteins, cyclin D1, VEGF, and ICAM-1.These results suggest that LCA has potential therapeutic activity in suppression of CCA cells.
Details
- ISSN :
- 2476762X
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- Asian Pacific Journal of Cancer Prevention
- Accession number :
- edsair.doi.dedup.....3e05e5d4045736fbe94eb14d62b14627