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Role of NADH: quinone oxidoreductase-1 in the tight junctions of colonic epithelial cells
- Source :
- BMB Reports, BMB REPORTS(47): 9
- Publication Year :
- 2014
- Publisher :
- Korean Society for Biochemistry and Molecular Biology, 2014.
-
Abstract
- NADH:quinone oxidoreductase 1 (NQO1) is known to be involved in the regulation of energy synthesis and metabolism, and the functional studies of NQO1 have largely focused on metabolic disorders. Here, we show for the first time that compared to NQO1-WT mice, NQO1-KO mice exhibited a marked increase of permeability and spontaneous inflammation in the gut. In the DSS-induced colitis model, NQO1-KO mice showed more severe inflammatory responses than NQO1-WT mice. Interestingly, the transcript levels of claudin and occludin, the major tight junction molecules of gut epithelial cells, were significantly decreased in NQO1-KO mice. The colons of NQO1-KO mice also showed high levels of reactive oxygen species (ROS) and histone deacetylase (HDAC) activity, which are known to affect transcriptional regulation. Taken together, these novel findings indicate that NQO1 contributes to the barrier function of gut epithelial cells by regulating the transcription of tight junction molecules. [BMB Reports 2014;47(9): 494-499]
- Subjects :
- Histone acetylation/deacetylation
Cell Membrane Permeability
Down-Regulation
Inflammation
Biology
Occludin
Quinone oxidoreductase
Biochemistry
Histone Deacetylases
Tight Junctions
Mice
Chromosome condensation
Claudin-1
medicine
Transcriptional regulation
NAD(P)H Dehydrogenase (Quinone)
Animals
Claudin
Molecular Biology
Research Articles
Gut inflammation
Cells, Cultured
chemistry.chemical_classification
Mice, Knockout
Reactive oxygen species
NQO1 knockout mice
Tight junction
Epithelial Cells
General Medicine
Colitis
Gut epithelial cell tight junction
Cell biology
Disease Models, Animal
Barrier dysfunction of epithelial cells
Histoneacetylation/deacetylation
Transcription
chemistry
Histone deacetylase
medicine.symptom
Reactive Oxygen Species
Subjects
Details
- Language :
- English
- ISSN :
- 1976670X and 19766696
- Volume :
- 47
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- BMB Reports
- Accession number :
- edsair.doi.dedup.....3df0d64cec7100311c8607f7b471fe7c