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Multicenter Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Study for Identification of Clinically Relevant Nocardia spp
- Source :
- Journal of clinical microbiology. 54(5)
- Publication Year :
- 2015
-
Abstract
- This multicenter study analyzed Nocardia spp., including extraction, spectral acquisition, Bruker matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) identification, and score interpretation, using three Nocardia libraries, the Bruker, National Institutes of Health (NIH), and The Ohio State University (OSU) libraries, and compared the results obtained by each center. A standardized study protocol, 150 Nocardia isolates, and NIH and OSU Nocardia MALDI-TOF MS libraries were distributed to three centers. Following standardized culture, extraction, and MALDI-TOF MS analysis, isolates were identified using score cutoffs of ≥2.0 for species/species complex-level identification and ≥1.8 for genus-level identification. Isolates yielding a score of Nocardia MALDI-TOF MS library with both the OSU and NIH libraries increased the genus-level and species-level identification by 18.2% and 36.9%, respectively. Overall, this study demonstrates the ability of diverse clinical microbiology laboratories to utilize MALDI-TOF MS for the rapid identification of clinically relevant Nocardia spp. and to implement MALDI-TOF MS libraries developed by single laboratories across institutions.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Veterinary medicine
Pathology
medicine.medical_specialty
030106 microbiology
Nocardia Infections
Matrix assisted laser desorption ionization time of flight
Biology
Mass spectrometry
Sensitivity and Specificity
Nocardia
03 medical and health sciences
medicine
Bacteriological Techniques
Ms analysis
Reproducibility of Results
Mycobacteriology and Aerobic Actinomycetes
Score interpretation
biology.organism_classification
United States
Clinical microbiology
Multicenter study
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Subjects
Details
- ISSN :
- 1098660X
- Volume :
- 54
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Journal of clinical microbiology
- Accession number :
- edsair.doi.dedup.....3de6c0fefa465cb28eef58065c1b101f