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Can the Genetic Origin Affect Rabbit Seminal Plasma Protein Profile along the Year?
- Source :
- Impreso, ReDivia. Repositorio Digital del Instituto Valenciano de Investigaciones Agrarias, instname
- Publication Year :
- 2015
-
Abstract
- The study was designed to evaluate the influence of genetic origin on rabbit seminal plasma protein profile variation along the year. Seminal plasma of rabbits from line A (maternal line) and R (paternal line) collected during a natural year was subjected to polyacrylamide gel electrophoresis (SDS-PAGE). The electrophoretic profile of rabbit seminal plasma resulted in multiple protein bands of different intensity ranging from 9 to 240 kDa. Results showed that seven protein bands were significantly different between genetic lines, and among these, three protein bands were significantly different between seasons. The differentially expressed proteins were identified by MALDI-TOF/TOF or LC-MS/MS analysis and were the following ones: FAM115E-like (220, 113 and 59 kDa), ectonucleoside triphosphate diphosphohydrolase 3 isoform X2 (72 kDa), annexin A5 (32 kDa), lipocalin allergen Ory c 4 precursor (19 kDa), and haemoglobin subunit zetalike (13 kDa) between genetic lines and FAM115E-like (113 kDa), haemoglobin subunit zetalike (13 kDa) and β-nerve growth factor (12 kDa) between seasons. These results indicate that proteins from rabbit seminal plasma are under both seasonal control and genetic control. Furthermore, the differential presence of these proteins could be one of the causes explaining the differences observed in fertility and seminal parameters between these two lines in earlier studies.
- Subjects :
- Gene isoform
Male
Seminal Plasma Proteins
Protein subunit
Semen
Biology
Lipocalin
03 medical and health sciences
0302 clinical medicine
Endocrinology
Blood plasma
Animals
Polyacrylamide gel electrophoresis
Genetics
030219 obstetrics & reproductive medicine
0402 animal and dairy science
04 agricultural and veterinary sciences
040201 dairy & animal science
Blood proteins
Molecular biology
Semen Analysis
Gene Expression Regulation
Animal Science and Zoology
Rabbits
Seasons
Biotechnology
Subjects
Details
- ISSN :
- 14390531
- Volume :
- 51
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Reproduction in domestic animals = Zuchthygiene
- Accession number :
- edsair.doi.dedup.....3dd3ddda2daf6656e70110d712e0e9d5