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Integrative analysis of DNA methylation, mRNAs, and small RNAs during maize embryo dedifferentiation
- Source :
- BMC Plant Biology, BMC Plant Biology, Vol 17, Iss 1, Pp 1-12 (2017)
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- Background Maize (Zea mays) is an important model crop for transgenic studies. However, genetic transformation of maize requires embryonic calli derived from immature embryo, and the impact of utilizing tissue culture methods on the maize epigenome is poorly understood. Here, we generated whole-genome MeDIP-seq data examining DNA methylation in dedifferentiated and normal immature maize embryos. Results We observed that most of the dedifferentiated embryos exhibited a methylation increase compared to normal embryos. Increased methylation at promoters was associated with down-regulated protein-coding gene expression; however, the correlation was not strong. Analysis of the callus and immature embryos indicated that the methylation increase was induced during induction of embryonic callus, suggesting phenotypic consequences may be caused by perturbations in genomic DNA methylation levels. The correlation between the 21-24nt small RNAs and DNA methylation regions were investigated but only a statistically significant correlation for 24nt small RNAs was observed. Conclusions These data extend the significance of epigenetic changes during maize embryo callus formation, and the methylation changes might explain some of the observed embryonic callus variation in callus formation. Electronic supplementary material The online version of this article (doi:10.1186/s12870-017-1055-x) contains supplementary material, which is available to authorized users.
- Subjects :
- 0301 basic medicine
DNA, Plant
Callus formation
MeDIP_seq
Plant Science
Biology
Zea mays
Epigenesis, Genetic
Epigenome
03 medical and health sciences
lcsh:Botany
Gene expression
Immunoprecipitation
RNA, Messenger
Epigenetics
Promoter Regions, Genetic
Genetics
24 nt small RNAs
fungi
food and beverages
Embryo
Embryo callus
Sequence Analysis, DNA
Methylation
DNA Methylation
lcsh:QK1-989
Maize
Cell biology
030104 developmental biology
RNA, Plant
Callus
Seeds
DNA methylation
Genome, Plant
Research Article
Subjects
Details
- ISSN :
- 14712229
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- BMC Plant Biology
- Accession number :
- edsair.doi.dedup.....3dc3a8d1a0e241c43391a4c5853e736b